化疗药物对原代胃癌细胞的体外杀伤效应及其与Bcl－2表达的关系  

Anti-tumor effect of chemotherapeutic drugs on human gastric cancer cells in vitro and the relationship with Bcl-2 expression

作　　者：耿明[1] 尹迎春[1] 曹永成[1] 付质杰[1] 邰艳红[1] 

机构地区：[1]济南军区总医院病理科,济南250031

出　　处：《中华胃肠外科杂志》2008年第3期276-279,共4页Chinese Journal of Gastrointestinal Surgery

摘　　要：目的观察化疗药物对原代胃癌细胞的体外杀伤作用，探讨其与胃癌组织中Bcl-2蛋白表达的关系。方法新鲜胃癌组织制备单细胞悬液，分别加入紫杉醇（Tax）、顺铂（DDP）、阿霉素（ADM）、氟尿嘧啶（5-Fu）和丝裂霉素（MMC）培养48h。四氮唑盐还原法（MTr）观察药物作用后癌细胞活力及代谢活性变化。免疫组织化学技术检测Bcl-2的表达。结果5种化疗药物平均抑制率依次为Tax（40．6±6．9）％、5．FU（38．9±9．2）％、CDDP（38．4±7．8）％、ADM（31．6±8．5）％、MMC（28．9±9．8）％。不同类型胃癌对5种化疗药物的敏感性由强到弱依次为印戒细胞癌、管状腺癌、黏液腺癌和乳头状腺癌。全组Bcl-2阳性率为80％，阳性者对5-FU、MMC和ADM有较强的耐药性。结论MTr比色法体外药敏试验。有助于筛选个体化有效治疗药物。Bcl-2的高表达可能是胃癌多药耐药的原因之一。Objective To evaluate in vitro anti-tumor effect of chemotherapeutic drugs on human gastric cancer cells, and investigate the relationship with Bcl-2 expression. Methods Single cell suspension was prepared from fresh gastric cancer tissue and exposed to taxol （Tax）, 5-fluorouracil （5-FU）, cisplatin （CDDP）, adriamycin （ADM）, mitomycin （MMC） respectively for 48 hours. Metabolic activity and inhibitory rate of cells were detected by MTT assay. Expression of Bcl-2 was examined with immunohistochemistry. Results The inhibitory rates of cancer cells exposed to chemotherapeutic drugs were different and Tax, 5-FU, CDDP had remarkabely higher rates than ADM and MMC. The lower differentiated gastric cancer cells were more sensitive than the higher ones. Positive expression rate of Bcl-2 was 80% and the positive cells showed resistance to 5-FU, ADM and MMC. Conclusions Chemosensitive testing by MTT assay can constitute the prediction for the application of chemotherapeutic drugs individually. Overexpression of Bcl-2 may contribute to multiple drug-resistance of tumors.

关 键 词：胃肿瘤 基因 BEL-2 化疗药物 敏感性 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤] R730.53[医药卫生—临床医学]