胰腺癌组织Survivin的表达与细胞凋亡、增殖的关系  

Relationship between Survivin expression and indexes of cel apoptosis and proliferation in pancreatic carcinoma

作　　者：倪金良[1] 陈晓星[1] 张国新[1] 郝波[1] 施瑞华[1] 王学浩[2] 

机构地区：[1]南京医科大学第一附属医院消化科,江苏省南京市210029 [2]南京医科大学第一附属医院肝脏外科,江苏省南京市210029

出　　处：《世界华人消化杂志》2008年第13期1425-1429,共5页World Chinese Journal of Digestology

摘　　要：目的:观察胰腺癌组织Survivin的表达与细胞凋亡、增殖的关系.方法:采用TUNEL法和免疫组化法检测64例胰腺癌及癌旁组织的细胞凋亡指数(AI)与增殖指数(PI)及Survivin表达,分析AI、PI与Survivin表达和临床病理因素的相关性.结果:Survivin蛋白在癌旁组织无表达,在胰腺癌组织阳性率为87.9%,两者比较差异显著(P<0.001).AI与年龄、性别及肿瘤部位无关,随胰腺癌组织分化程度的增加而增加,癌旁组织显著高于胰腺癌组织(30.47%vs1.64%,P<0.001);PI与年龄、性别、肿瘤部位及淋巴结转移无关,而与癌组织的分化程度相关,随着胰腺癌分化程度的降低而增加.从分化程度观察,胰腺癌组织AI与Survivin表达水平呈负相关(r=-0.85),PI与Survivin表达水平呈正相关(r=0.87);从有无转移来看,胰腺癌AI与Survivin表达水平呈负相关(r=-0.81).结论:Survivin凋亡抑制基因在胰腺癌发生和发展中起重要作用.AIM： To investigate the relationship between survivin expression and the indexes of apoptosis and proliferation in pancreatic carcinoma tissues. METHODS： Sixty-four pairs of pancreatic carcinoma and cancer-adjacent tissues were included in this study. TUNEL method was used to measure the cell apoptosis index, and immunohistochemistry was used to detect ki-67 expression （standing for the cell proliferation index） and Survivin expression. The relations of apoptosis index and proliferation index with Survivin expression and pathological features were analyzed. RESULTS： The apoptosis index had no correla-tion with the age and gender of patients and tumor location, increased with the elevation of carcinoma differentiated degrees, and was markedly higher in cancer-adjacent tissues than that in cancer tissues （30.47% vs 1.64%, P 〈 0.001）. The proliferation index had no correlation with the age and sex of patients, tumor location and lymphatic metastases, increased with the reduction of carcinoma differentiated degrees. From the view of differentiation, Survivin expression was negatively correlated with the apoptosis index （r = -0.85）, but positively correlated with the proliferation index （r = 0.87） in pancreatic carcinoma tissues. From the view of metastases, the apoptosis index was negatively correlated with Survivin expression （ = -0.81）. CONCLUSION： Survivin gene plays an important role in the pathogenesis and development of pancreatic carcinoma.

关 键 词：胰腺癌 SURVIVIN Ki-67 凋亡指数 增殖指数 癌旁组织 免疫组化 TUNEL法 

分 类 号：R735.9[医药卫生—肿瘤]