神经生长因子的脑保护作用与Caspase-3表达的相关性  

Relationship between the therapeutic window of NGF and the expression of Caspase-3

作　　者：杨冀萍[1] 刘新峰[1] 刘怀军[2] 刘瑞春[3] 李春岩[3] 

机构地区：[1]南京军区南京总医院神经内科,210002 [2]河北医科大学第二医院医学影像科 [3]河北医科大学第二医院神经内科

出　　处：《中华神经外科杂志》2008年第5期390-392,共3页Chinese Journal of Neurosurgery

基　　金：国家自然科学基金项目（30470604）;中国博士后基金项目（20070411051）

摘　　要：目的研究神经生长因子（NGF）的脑保护时间窗与半胱天冬酶-3（Caspase-3）表达的相关性。方法采用兔局灶性脑缺血再灌注损伤模型，分别于再灌注后Oh、1h、3h和6h将NGF立体定向导入梗死灶周，再灌注72h观察神经功能、梗死体积、灶周凋亡率和Caspase-3表达。结果再灌注0h、1h和3h灶周给予NGF，梗死体积分别较对照组下降50．1％、42．5％和35．2％，相应的灶周凋亡率及Caspase-3表达明显下降，神经功能恢复较好，用药越早越明显；再灌注6h给药，则无明显作用。相关分析显示梗死体积变化与Caspase-3表达具有明显相关性（P〈0．05）。结论NGF脑保护治疗时间窗与Caspase-3表达相关，抑制Caspase-3表达可能是NGF介导神经保护作用的机制之一。Objective To study the relationship between the therapeutic window of opportunity for NGF following focal cerebral ischemia-reperfusion injury in rabbits and the expression of cysteinyl aspirate- specific proteinase-3 （Caspase-3） protein. Method Focal cerebral ischemia-reperfusion model in rabbit was induced. NGF was administered at 0, 1, 3 and 6h after reperfusion respectively. Infarct volume, neurological deficit score, apoptotic percentage, and expression of Caspase-3 were assessed at 72h after reperfusion. Results NGF reduced infarct volumes compared with saline control group by 50. 1%, 42.5% and 35.2% when administered NGF at 0, 1 and 3h after reperfusion. The percentage of apoptotic cell and expression of Caspase-3 in the perifocal regions were significantly lower than that in control group when administered NGF within 3h after reperfusion （P 〈 0.01）. There was no difference when administered NGF at 6h after reperfusion （ P 〈 0. 05 ）. Infarct volumes were correlated significantly with expression of Caspase- 3 in the perifocal regions （ P 〈 0.01 ）. Conclusions The effect of NGF on the expression of Caspase-3 in perifocal regions is related with the therapeutic time window. NGF-induced neuroprotective effect may be involved in reducing the expression of Caspase-3 protein.

关 键 词：神经生长因子 缺血再灌注损伤 半胱天冬酶-3 时间窗 

分 类 号：R741[医药卫生—神经病学与精神病学]