应用人大肠癌肝转移裸鼠模型抗转移药物筛选研究  

SCREENING ANTI METASTASIS DRUGS AGAINST COLORECTAL CARCINOMA BY USING LIVER METASTASIS MODEL IN NUDE MICE

作　　者：朱梅刚[1] 邱红明[1] 杨学皆[1] 卢凯 邓永健[1] 张进华[1] 

机构地区：[1]第一军医大学病理学教研室

出　　处：《中国癌症杂志》1997年第3期215-217,共3页China Oncology

摘　　要：目的建立抗人大肠癌肝转移动物模型，快速筛选高效低毒抗肝转移药物。方法对癌细胞血道转移的主要环节选定４种药物进行抗肝转移实验：①亚硒酸钠、蝎毒：两者均具有抗癌细胞侵袭能力；②虎杖Ⅳ号：具有抗癌细胞在微血管的粘附作用；③鱼精蛋白：为血管生成抑制剂。在人大肠癌细胞株脾脏植入肝转移裸鼠模型进行药物筛选实验研究。结果应用４种药物后的肝转移灶数明显少于对照组（Ｐ＜０．０１～Ｐ＜０．００５），显示抗肝转移效果。无肝、肾、心、肺等病理改变。结论４种药物均有明显的抗人大肠癌实验性肝转移效果。PURPOSE To establish a liver metastasis from human colorctal carcinoma (CRC) animal model for rapid screening of high efficacy low toxicity anti liver metastasis drug. METHODS Four drugs were selected based on the process of hematogenous metastasis of CRC. They were:(1)Na 2Se0 3 and scorpium toxin, both of which have a suppressive effect on invasiveness of cancer cells;(2)polygonus caspidatus which could suppress the adhesion of tumor cells to endotherlial cells in the microcirculation of target organs;(3)salmine sulphate which could inhibit angiogenesis in the tumor.These four drugs were screened by using liver metastasis model in nude mice. RESULTS The results showed that all drugs had significant effects as anti metastatic agents in CRC on liver metastasis in the nude mice models without observable side effects on the liver, kidney, brain etc. CONCLUSIONS All four drugs were effective in the experimental study. No significant differences in anti metastatic efficacy were observed between each drug( P >0 05)

关 键 词：结直肠肿瘤 肝转移 肿瘤转移 疾病模型 药物筛选 

分 类 号：R735.340.6[医药卫生—肿瘤] R979.1[医药卫生—临床医学]