玻璃体内注射先锋霉素Ⅵ视网膜酶组织化学的实验研究  

AN EXPERIMENTAL STUDY OF ENZYMOHISTOCHEMISTRY IN RETINA BY INTRAVITREAL INJECTION OF CEPHRADINE

作　　者：牛膺筠[1] 周占宇[1] 纪建中[1] 林红[1] 程丹富[1] 杨文毅 

机构地区：[1]青岛医学院附属医院眼科

出　　处：《中华眼底病杂志》1997年第3期139-142,共4页Chinese Journal of Ocular Fundus Diseases

摘　　要：目的：研究先锋霉素Ⅵ玻璃体腔内注射的安全剂量。方法：不同浓度的先锋霉素Ⅵ（１００、２００、２５０、３００、４００μｇ）注入不同组别的兔眼玻璃体腔内，以酶组织化学染色法测定注射后不同时间（１、３、７天）视网膜能量代谢酶琥珀酸脱氢酶（ｓｕｃｃｉｎｉｃｄｅｈｙｄｒｏｇｅｎａｓｅ，ＳＤＨ）和乳酸脱氢酶（ｌａｃｔｉｃｄｅｈｙｄｒｏｇｅｎａｓｅ，ＬＤＨ）活性的变化并观察视网膜的组织结构及超微结构损害。结果：随用药剂量增大，视网膜内ＳＤＨ、ＬＤＨ活性均逐渐降低；各剂量组活性分别于术后３天和术后１天降至最低，然后开始恢复，至术后７天１００、２００μｇ组恢复至正常，其余剂量组仍低于正常。１００、２００μｇ剂量组视网膜内仅出现水肿，而２５０、３００、４００μｇ组均出现细胞变性坏死、杆锥体脱失等改变。结论：先锋霉素Ⅵ玻璃体腔内注射的安全剂量为２００μｇ。OBJECTIVE:To verify the safe dose of cephradine in intravitreal injection.METHODS:After injecting different doses of cephradine(100μg,200μg,250μg,300μg,400μg)into vitreous cavity of different group of rabbits the activities of the retinal enzymes(SDH,LDH)on different time(1d、3d,7d) were determined respectively,and the histological and ultrastructural changes of retinas were also observed simultaneously.RESULTS:The activity of retinal SDH and LDH was found to be decreased gradually with the icreasing of the dosage of intravitreal cephradine.The activities of SDH and LDH were found in the lowest level on the 3rd and lst day,but they recover to normal levels on the 7th day after intravitreal injection in 100μg,200μg groups,and still lower than normal in the other groups.Histologically,retinal edema was found both in 100μg and 200μg groups,but degradation of retinal cells,and loss of cones and rods were found in the 250μg,300μg and 400μg groups.CONCLUSION: The safe dose of intravitreal injection of cephradine is 200μg.

关 键 词：头孢拉定 剂量 视网膜 酶学 玻璃体 组织化学 

分 类 号：R978.11[医药卫生—药品] R451[医药卫生—药学]