Assembly of the Protoplasm of Codium fragile (Bryopsidales, Chlorophyta) into New Protoplasts  

作　　者：Demao Li Fang Lu Guangce Wang Baicheng Zhou 

机构地区：[1]Institute of Oceanology, the Chinese Academy of Sciences, Qingdao 266071, China [2]College of Manne Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China [3]Department of Food Science, College of Agriculture, Liaocheng University, Liaocheng 252059, China [4]Graduate School, the Chinese Academy of Sciences, Beijing 100039, China

出　　处：《Journal of Integrative Plant Biology》2008年第6期752-760,共9页植物学报（英文版）

基　　金：the Project for Supporting the National Development(2006BAD09A04);the Hi-Tech Research and Development (863) Program of China (2006AA05Z112 and 2006AA10A413);grants from the NationalNatural Science Foundation of China (U0633006).

摘　　要：The cell organelles of the coenocytic alga Codium fragile （Sur.） Harlot aggregated rapidly and protoplasts were formed when its protoplasm was extruded out in seawater. Continuous observation showed that there were long and gelatinous threads connecting the cell organelles. The threads contracted, and thus the cell organelles aggregated into protoplasmic masses. The enzyme digestion experiments and Coomassie Brilliant Blue and Anthrone stainings showed that the long and gelatinous threads involved in the formation of the protoplasts might include protein and saccharides as structure components. Nile Red staining indicated that the protoplast primary envelope was non-lipid at first, and then lipid materials integrated into its surface gradually. The fluorescent brightener staining indicated that the cell wall did not regenerate in the newly formed protoplasts and they all disintegrated within 72 h after formation. Transmission electron microscopy of the cell wall of wild C. fragile showed electron-dense material embedded in the whole cell wall at regular intervals. The experiments indicated that C. fragile would be a suitable model alga for studying the formation of protoplasts.The cell organelles of the coenocytic alga Codium fragile （Sur.） Harlot aggregated rapidly and protoplasts were formed when its protoplasm was extruded out in seawater. Continuous observation showed that there were long and gelatinous threads connecting the cell organelles. The threads contracted, and thus the cell organelles aggregated into protoplasmic masses. The enzyme digestion experiments and Coomassie Brilliant Blue and Anthrone stainings showed that the long and gelatinous threads involved in the formation of the protoplasts might include protein and saccharides as structure components. Nile Red staining indicated that the protoplast primary envelope was non-lipid at first, and then lipid materials integrated into its surface gradually. The fluorescent brightener staining indicated that the cell wall did not regenerate in the newly formed protoplasts and they all disintegrated within 72 h after formation. Transmission electron microscopy of the cell wall of wild C. fragile showed electron-dense material embedded in the whole cell wall at regular intervals. The experiments indicated that C. fragile would be a suitable model alga for studying the formation of protoplasts.

关 键 词：Codium fragile PROTOPLASM PROTOPLAST cell wall 

分 类 号：Q943[生物学—植物学]