血清MUC1、MUC2及MUC5AC表达与胃癌的关系  

Relationship between serum expression of MUC1,MUC2 and MUC5AC and gastric cancer

作　　者：黄晓辉[1] 徐迎新[1] 李荣[1] 刘洪一[1] 张敏丽[2] 

机构地区：[1]中国人民解放军总医院普通外科研究所,北京市100853 [2]中国人民解放军军事医学科学院,北京市100850

出　　处：《世界华人消化杂志》2008年第12期1299-1303,共5页World Chinese Journal of Digestology

基　　金：国家自然科学基金面上资助项目;No.30070736~~

摘　　要：目的:探讨胃癌血清中MUC1、MUC2及MUC5AC与胃癌的关系.方法:利用黏蛋白MUC1、MUC2及MUC5AC的单抗及多抗,制备黏蛋白芯片,用免疫荧光原理进行检测,并用CEA作为指示指标,对30例胃癌患者及30例健康人进行黏蛋白血清水平的检测.结果:胃癌组与对照组相比,3种黏蛋白血清水平均增高,两组间差异显著.MUC1的阳性表达与胃癌TNM分期相关(P=0.0047),MUC2和MUC5AC的阳性表达与TNM分期无关(P=0.136,P=0.201).3种黏蛋白的表达均随胃癌分化程度降低而阳性表达增高的趋势.各单一黏蛋白以及黏蛋白两两之间联合用于胃癌诊断的敏感性及特异性均较高,其中单一黏蛋白中,MUC1的的敏感性和特异性最好,分别达到81.5%、75.8%,联合诊断(3种黏蛋白中至少2种阳性)的敏感性和特异性达到96.0%、82.9%.结论:检测血清黏蛋白MUC1、MUC2及MUC5AC水平,可以提高胃癌诊断的敏感性及特异性,为早期诊断提供新的思路,并对判断分期及预后提供帮助.AIM： To explore the association of mucins （MUC1, MUC2 and MUC5AC） expression in serum with gastric cancer. METHODS： Mucin protein chip was made using monoclonal and multiclonal antibodies and then applied to measure the levels of serum MUC1, MUC2 and MUC5AC in 30 gastric cancer patients and 30 healthy adults using the method of immunofluorescence （carcinoembryonic antigen as an indicator）. RESULTS： The levels of serum mucins and CEA in gastric cancer patients were significantly higher than those in the healthy controls. The positive expression of MUC1 was related with the TNM staging for gastric cancer （P = 0.0047）,but the positive rates of MUC2 and MUC5AC expression had no relationship with the TNM staging （P = 0.136, P = 0.201）. The positive rates of mucins expression were increased with the descending of cancer differentiation degree. Single mucin or either two mucins were sensitive and specific in the diagnosis of gastric cancer. For single mucin detection, MUC1 was the most sensitive and specific, with a sensibility of 81.5% and a specificity of 75.8%, respectively. Mucins in combination （at least 2 positive） had a sensibility of 96.0% and a specificity of 82.9%, respectively. CONCLUSION： Serum mucins detection has higher sensibility and specificity in the diagnosis of gastric cancer, which may provide a new approach to early diagnose and prognose gastric cancer.

关 键 词：黏蛋白 胃癌 蛋白芯片 

分 类 号：R735.2[医药卫生—肿瘤] R737.31[医药卫生—临床医学]