TGF-α和Cyclin E在中医不同证型患者胃病变组织中的表达及相关性  

Expression of transforming growth factorα and Cyclin E and their correlation in chronic gastric lesion tissues from patients with different TCM types

作　　者：梁卫江[1] 罗荣城[1] 马业俊[2] 阎曦[2] 张万岱[2] 

机构地区：[1]南方医科大学南方医院肿瘤中心,广东省广州市510515 [2]南方医科大学南方医院消化科,广东省广州市510515

出　　处：《世界华人消化杂志》2008年第12期1355-1358,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨中医不同证型患者的慢性胃病变组织中TGF-α和CyclinE表达的变化,并分析两者表达的相关性.方法:慢性胃病患者(慢性良性胃病变和胃癌)135例,按中医分为肝胃不和、脾胃虚寒、胃阴不足和寒热夹杂4种证型.对患者慢性胃病变组织进行TGF-α和CyclinE表达的免疫组化染色,对两者在各中医证型中表达的阳性率进行比较,并进一步分析TGF-α和CyclinE表达的相关性.结果:TGF-α在4种中医证型中表达的阳性率分别为25.0%、18.6%、47.1%和42.1%,在胃阴不足型与脾胃虚寒型中的表达,有显著性差异(P<0.05).CyclinE表达的阳性率则分别为20.0%、7.0%、26.5%和31.6%,在寒热夹杂型与脾胃虚寒型中表达的,有显著性差异(P<0.05).TGF-α和CyclinE表达均在脾胃虚寒型最低.TGF-α和CyclinE表达存在显著的正相关关系.结论:在中医不同证型患者中,TGF-α和CyclinE在胃病变组织中的表达存在差异,TGF-α和CyclinE的表达存在正相关关系.AIM： To explore the expression of transforming growth factor α （TGF-α） and Cyclin E in chronic gastric lesion tissues from patients with different types of traditional Chinese medicine （TCM）, and analyze the correlation between TGF-α and Cyclin E expression. METHODS： The patients （n = 135） with chronic gastric diseases were classified to 4 groups according to different TCM types such as disharmony between liver and stomach （group A）, spleen-stomach cold deficiency （group B）, stomach-yin deficiency （group C）, and mixed cold and heat （group D）. Immunohistochemical staining was used to examine the expression of TGF-α and Cyclin E in the lesion tissues and the correlation between TGF-α and Cyclin E expression was also ssessed. RESULTS： The positive rates of TGF-α expression in the lesion tissues of group A, B, C and D were 25.0%, 18.6%, 47.1% and 42.1%, respectively, and there was significant difference between group B and C （P 〈 0.05）. The positive rates of Cyclin E expression were 20.0%, 7.0%, 26.5% and 31.6%, respectively, and there was marked difference between group B and D （P 〈 0.05）. Both TGF-α and Cyclin E expression were the lowest in group B, and there existed a positive correlation between the expression of TGF-α and Cyclin E. CONCLUSION： TGF-α and Cyclin E are differentially expressed in chronic gastric lesion tissues of patients with different TCM types, and the expression of TGF-α and Cyclin E are positively correlated.

关 键 词：慢性胃疾病 中医证型 转化生长因子Α CYCLIN E 

分 类 号：R259[医药卫生—中西医结合]