GC-MS联用分析测定志贺菌全细胞脂肪酸组成  

GC-MS analysis of fatty acid from whole cell of Shigella

作　　者：秦巧玲[1] 粟婉媛[1] 吕均[1] 郭爱玲[1] 

机构地区：[1]华中农业大学食品科学技术学院,武汉430070

出　　处：《中国卫生检验杂志》2008年第3期426-428,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:通过气相色谱-质谱法分析志贺菌全细胞脂肪酸,建立志贺菌全细胞脂肪酸的指纹图谱,为志贺菌的鉴定提供依据。方法:用气相色谱-质谱法分析了4种志贺菌的全细胞脂肪酸。结果:志贺菌全细胞脂肪酸主要脂肪酸有C12,C13,C14,C15,C16,C17,C18:1。志贺菌属脂肪酸成分都具有一定的特征,4个种之间也表现出一定的差异,标准菌株与地方菌株极其相似,同种不同来源的地方菌株表现出一定差异。结论:本结果为志贺菌的化学分类和快速鉴定提供参考资料。Objective： By gas chromatogram - mass spectrograph （ GC - MS）, the whole cellular fatty acids of Shigella are analysed. In addition, the fingerprint of the componentsof cellular fatty acids of Shigella are set up and these provide basis for the identification of the Shigella. Methods： The whole cellular fatty acids of 4 species of Shigella were analyzed by gas chromatogrammass spectrograph （GC -MS）. Results：The fatty acids of the Shigella were mainly C12, C13, C14, C15, C16, C17, C18：1. The whole cellular fatty acids of Shigellas species were quite similar. The components of fatty acid from different species of Shigella were different, but it was the same in type strains and local strains. Conclusion： This results can be used as a rapid identification method for Shigella.

关 键 词：志贺菌 气相色谱-质谱法 细胞脂肪酸 

分 类 号：O657.63[理学—分析化学]