苦参素对胃癌SGC-7901细胞凋亡及细胞骨架改变的研究  

Study of the effects of oxymatrine on apoptosis and changes of cytoskeleton of human gastric carcinoma SGC-7901 cells

作　　者：高喜仁[1] 何军[1] 王丽芳[1] 

机构地区：[1]齐齐哈尔医学院解剖教研室,161042

出　　处：《齐齐哈尔医学院学报》2008年第8期905-906,共2页Journal of Qiqihar Medical University

基　　金：黑龙江省教育厅资助项目;齐齐哈尔科技局资助项目

摘　　要：目的探讨苦参素对胃癌细胞株SGC-7901的抑制增殖效应及凋亡诱导作用。方法用苦参素处理SGC-7901细胞,采用MTT法检测苦参素对细胞的抑制作用,透射电镜观察微丝的变化,流式细胞术检测细胞凋亡率和细胞周期。结果苦参素使SGC-7901细胞呈凋亡改变,细胞的活力下降,微丝几近消失,不同浓度的苦参素均可导致细胞凋亡和细胞周期阻抑。结论苦参素能抑制胃癌细胞增殖,能诱导胃癌细胞凋亡。Objective To explore the effects of Oxymatrine on the proliferative inhibition and apoptosis induction of paclitaxel of Human gastric carcinoma SGC-7901 cells. Methods SGC-7901 cells were treated with Oxymatrine. The inhibitory effects of Oxymatrine were detected by MTT method. The changes of the degradation process were observed by transmission electron microscopy. Apoptosis rates and cell cycle were determined by flow cytometry. Results Oxymatrine made SGC-7901 cells present apoptotic changes, and the cytoactives were evidently declined, the degradation process almost disappeared. The oxymatrine of different dent icy can cause cell Apoptosis and cell cycle represses. Conclusions Oxymatrine can inhibit proliferation and promote the apoptosis of gastric carcinoma cells.

关 键 词：苦参素 细胞凋亡 胃癌细胞株 细胞骨架 

分 类 号：R735.2[医药卫生—肿瘤] R733.71[医药卫生—临床医学]