HPLC法测定牛奶中游离唾液酸和与低聚糖结合的唾液酸含量  

Determination of Contents of Free and Oligosaccharide-bound Sialic Acid in Milk by HPLC

作　　者：冯君[1] 杨国宇[1] 李宏基[1] 韩立强[1] 林茂旺[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《食品科学》2008年第5期355-357,共3页Food Science

基　　金：河南省重点科技攻关项目(0522010800)

摘　　要：利用酸水解法把牛奶中的唾液酸释放出来,以邻苯二氨盐酸盐(10mg/ml)为衍生化试剂,在80℃水浴锅中衍生40min。采用Symmetry C18柱(250mm×4.6mm,5μm),流动相为1.0%的四氢呋喃水溶液(含0.2%磷酸)-乙腈(92:8);流速为1.0ml/min,柱温35℃,紫外检测波长为230nm。结果表明,唾液酸在10～320μg/ml范围内线性良好,平均回收率为95.7%。本方法具有灵敏度高,重复性好等特点,可准确测定牛奶中游离的唾液酸和与低聚糖结合的唾液酸含量。Sialic acid in milk was released by acid hydrolysis, and then was derivated with O-pheny-lenediamine： 2HCl （10mg/ ml）at 80℃ for 40 min. The conditions of HPLC were： Symmetry C18 column（250 mm × 4.6 mm, 511 m）, the mobile phase 1.0% tetraphdrofuran（containing 0.2 % phosphoric acid）-acetonitrile（92：8）, the flow rate 1.0ml/min, the column temperatue 35 ℃, and the detection wave length 230 nm. The results indicated that the linear rang of sialic acid was 10～320μg/ml. The average recovery was 95.7 %. The method has the characteristics of good reproducibility and high sensitivity. It can be used to determine the contents of the free and oligosaccharide-bound sialic acid in milk.

关 键 词：牛奶 N-乙酰神经氨酸 高效液相色谱法 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]