神经胶质瘤中生长抑制因子基因的表达  

Expression of ING4 gene in human glioma

作　　者：李铁臣[1] 朱延玲[2] 宫磊[1] 曹宏伟[1] 汪萌芽[3] 

机构地区：[1]皖南医学院分子生物学研究室,安徽省芜湖市241002 [2]安徽医学高等专科学校护理系,安徽省合肥市230601 [3]皖南医学院细胞电生理研究室,安徽省芜湖市241002

出　　处：《实用医学杂志》2008年第9期1508-1510,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金(编号:30270366)

摘　　要：目的:探讨生长抑制因子4(ING4)基因在神经胶质瘤发生发展过程中的作用。方法:采用RT-PCR方法扩增34例神经胶质瘤患者手术标本和24例正常脑组织标本的ING4基因和内参照GAPDH基因,凝胶分析软件对RT-PCR产物进行半定量分析,比较ING4基因mRNA在神经胶质瘤和正常脑组织中的表达情况。结果:所有的标本均扩增出ING4基因的目的条带。神经胶质瘤中的ING4基因mRNA表达水平明显低于正常脑组织(P<0.05)。恶性度高的神经胶质瘤组明显低于低度恶性神经胶质瘤组(P<0.05)。结论:ING4基因在神经胶质瘤的发生发展过程中可能起一定的作用,且与恶性程度有一定相关性。Objective To investigate the role of ING4 gene in the tumorigenesis and development of glioma.Methods The mRNA expressions of ING4 gene and intra-reference GAPDH gene in 34 specimens of glioma tissue and 24 of normal brain tissue were assessed by reverse transcription polymerase chain reaction （RT-PCR）, Results The target bands of ING4 gene were present in all of the specimens. The mRNA expression level of ING4 gene was significantly lower in glioma than in the normal brain tissue （P 〈 0.05）, and the level in the high-grade malignancy was markedly lower than that in the low-grade malignancy （P 〈 0.05）. Conclusion ING4 gene may play a role in the tumorigenesis and development of glioma and be related to the grade of the malignancy.

关 键 词：神经胶质瘤 ING4基因 表达 逆转录聚合酶链反应 

分 类 号：R739.4[医药卫生—肿瘤]