苏云金芽孢杆菌晶体蛋白对北方根结线虫生物测定方法的建立和杀虫效果评价(英文)  

Establish a Bioassay to Assess Nematicidal Effect of Bacillus thuringiensis Crystal Protein against Meloidogyne hapla

作　　者：余子全[1] 刘斌[1] 邹雪[1] 喻子牛[1] 孙明[1] 

机构地区：[1]华中农业大学生命科学技术学院,农业微生物学国家重点实验室,武汉430070

出　　处：《植物病理学报》2008年第2期219-224,共6页Acta Phytopathologica Sinica

基　　金：Key Project of China Programs for Fundamental Research and Development(973)(2003CB114201);;National Programs for High Technology Research and Development of China(863)(2006AA02Z174 and 2006AA03A243)

摘　　要：A bioassay procedure was developed to assess the toxicity of Bacillus thuringiensis crystal protein against Meloidogyne hapla,a root-knot nematode,under laboratory conditions.Reproducibility and precision of the bioassay results were optimal when forty 2nd stage juveniles were incubated in the dissolved crystal protein solution at 25℃,pH9.0 for 7 days.The juveniles were stained with 1% KMnO4 for 2 hours or methylene blue solution for 1 hour to distinguish living and dead ones.By the bioassay procedure,the LC50 value of strain YBT-1532 crystal protein against M.hapla was determined as 0.304±0.086 mg/mL(LC50±1.96SE).Moreover,the strain YBT-1532 showed toxicity to Caenorhabditis elegans,a free-living nematode.All results indicated that YBT-1532 is a toxic strain to plant-parasitic nematode,and has the potential to control plant-parasitic nematode.A bioassay procedure was developed to assess the toxicity of Bacillus thuringiensis crystal protein against Meloidogyne hapla, a root-knot nematode, under laboratory conditions. Reproducibility and precision of the bioassay results were optimal when forty 2^nd stage juveniles were incubated in the dissolved crystal protein solution at 25℃, pH9.0 for 7 days. The juveniles were stained with 1% KMnO4 for 2 hours or methylene blue solution for 1 hour to distinguish living and dead ones. By the bioassay procedure, the LC50 value of strain YBT-1532 crystal protein against M. hapla was determined as 0.304 ±0.086 mg/mL （LC50 ±1.96SE）. Moreover, the strain YBT-1532 showed toxicity to Caenorhabditis elegans, a free-living nematode. All results indicated that YBT-1532 is a toxic strain to plant-parasitic nematode, and has the potential to control plant- parasitic nematode.

关 键 词：苏云金芽孢杆菌 晶体蛋白 生物测定 测定方法 

分 类 号：S476.11[农业科学—农业昆虫与害虫防治] S859.795[农业科学—植物保护]