乙酰肝素酶mRNA在卵巢上皮性肿瘤中的表达及其临床病理相关性研究  

The relationship between the expression of heparanase mRNA and clinic-pathology of ovarian epithelial tumor

作　　者：韦露薇[1] 李力[1] 张玮[1] 黎丹戎[1] 潘忠勉[1] 唐步坚[1] 高琨[1] 

机构地区：[1]广西医科大学附属肿瘤医院妇瘤科,530021

出　　处：《中国妇产科临床杂志》2008年第3期196-198,共3页Chinese Journal of Clinical Obstetrics and Gynecology

摘　　要：目的探讨乙酰肝素酶（Hpa）mRNA在卵巢上皮性癌中的表达及与临床病理的相关性。方法应用逆转录-聚合酶链反应（RT-PCR）方法检测41例卵巢上皮性癌、17例卵巢良性肿瘤和22例正常卵巢组织中Hpa mRNA表达，并分析其与卵巢癌临床病理的相关性。结果①卵巢上皮性癌组织、良性肿瘤组织和正常组织Hpa mRNA阳性表达率分别为58．54％、29．41％和22．73％。癌组织Hpa mRNA阳性表达率显著高于良性组及正常组（P〈0．01），良性组与正常组Hpa mRNA阳性表达率比较，差异无显著性（P〉0．05）。②Ⅲ～Ⅳ期卵巢恶性肿瘤患者中Hpa mRNA的阳性表达率显著高于Ⅰ～Ⅱ期患者（P〈0．05）。低分化肿瘤组织中Hpa mRNA的阳性表达率显著高于中、高分化者（P〈0．05）。③Long-rank分析显示Hpa mRNA表达阳性者的累积生存率明显低于阴性者，差异有显著性（P〈0．05）。结论卵巢上皮性癌中Hpa mRNA阳性表达率明显增高，并与卵巢上皮性癌病情进展有关，可作为卵巢上皮性癌转移和预后的一个重要预测指标。Objective To explore the human heparanase （Hpa） mRNA expression and its relationship with the clinic-pathological characteristics in ovarian epithelial tumor. Methods Tissue specimens were collected from 41 ovarian epithelial carcinoma, 17 benign ovarian tumor and 22 normal ovaries. The Hpa mRNA expression was detected by reverse transcription polymerase chain reaction （RT - PCR）. Results ① The expression of Hpa rnRNA was significantly higher in ovarian cancer than in benign ovarian tumor and normal ovarian （58. 54% vs 29.41%, P〈0.01; 58. 54% vs 22. 73%, P〈0.05）. The expression of Hpa rnRNA was not significantly different between benign ovarian tumor and normal ovaries （P〉0.05） ; ② The expression of Hpa rnRNA was correlated with clinical stages and differentiation degree of cancer cells （P〈0.05） ; but not related to age, histological type, ascites, lymph node involvement and distant metastasis; ③ Expression of Hpa rnRNA was one of the risk factors of the prognosis （P〈0.05）. Conclusion Overexpression of the heparanase mRNA is related to development of ovarian cancer and might be an important marker of metastasis and prognosis.

关 键 词：乙酰肝素酶 卵巢上皮性癌 逆转录聚合酶链反应 

分 类 号：R737.31[医药卫生—肿瘤]