IL-1B及IL-1RN基因多态性与江苏地区宫颈癌遗传易感性相关  

Associations between functional genetic polymorphisms of IL-1B and IL-1RN and risk of cervical cancer

作　　者：钱年凤[1] 韩素萍[1] 陈小军[1] 朱慧[1] 王莉娜[2] 胡志斌[2] 沈洪兵[2] 

机构地区：[1]南京医科大学第一附属医院妇产科,江苏南京210029 [2]南京医科大学公共卫生学院肿瘤研究中心,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2008年第5期629-634,共6页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:探讨炎症因子IL-1B(T-31C和C-511T)及其拮抗基因IL-1RN基因多态性与宫颈癌遗传易感性的关系。方法:采用病例对照研究,对经组织学确诊的宫颈癌新发病例273例按年龄和城乡频数匹配正常对照402例,以聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法进行多态性检测,比较不同基因型与宫颈癌发病风险的关系。结果:在调整年龄、城乡等因素后,携带-511CT基因型比携带CC基因型个体发生宫颈癌的危险性增加93%(调整OR=1.93,95%CI=1.00～3.72);携带-511TT基因型比携带-511CC基因型个体发生宫颈癌的危险性增加115%(调整OR=2.15,95%CI=1.02～4.53)。分层分析表明,携带IL-1BT-31C变异基因型的个体发生宫颈癌的风险仅在年龄≥50岁(调整OR=1.72,95%CI=1.01～2.93)、妊娠次数≥2次(调整OR=1.83,95%CI=1.07～3.11)和农村地区女性(调整OR=1.63,95%CI=1.04～2.54)中显著。IL-1RN各基因型在病例组和对照组中分布的差异均无统计学意义。结论:IL-1BC-511T多态性可能与江苏地区妇女的宫颈癌易感性相关。Objective：To study the relationship between functional genetic variants of IL-1B （T-31C,C-511T） and IL-1RN genes and the susceptibility of cervical cancer. Methods：The case-control study was conducted in Jiangsu female population,including 273 cervical cancer cases and 402 cancer-free controls frequency-matched by age and residential areas. Genotypes were determined by PCR-the restriction length polymorphism（PCR-RFLP） assays. Results： The differences of IL-1B T-31C and C-511T genotype frequen- cies between cases and controls were not statistically significant. After adjusted age and residences, the Logistic regression analysis revealed that the increased risk of cervical cancer was associated with IL-1B -511TT and -511CT variant genotypes （OR=1.93,95% CI= 1.00-3.72 for CT genotypes ; OR=2.15,95% CI=1.02-4.53 for TT genotypes）, compared with the -511CC genotype. Stratified analysis indicated that IL-1B T-31C variant genotypes were statistically associated with cervical cancer risk in the subjects with more than 49 years old （OR=1.72,95%CI=1.01-2.93）,pregnancy frequency ≥2 （OR=1.83,95%CI=1.07-3.11） and living in countryside （OR= 1.63,95%CI=1.04-2.54）. No significant association was observed between polymorphisms of IL-1RN gene and cervical cancer risk. Conclusion：These findings supported the hypothesis that the functional genetic variants of IL-1B C-511T might contribute to the cervical carcinogenesis in Jiangsu population.

关 键 词：宫颈癌 IL-1B IL-1RN 单核苷酸多态性 分子流行病学 

分 类 号：R737.33[医药卫生—肿瘤]