乳腺癌肿瘤干细胞标记物CD44^+ESA^+CD24^(-Λow)在非小细胞肺癌组织中的表达及其意义  

Expression and Significance of CD44^+ESA^+CD24^(-Λow),Stem Cell Markers for Breast Cancer,in Non-small-cell Lung Carcinoma

作　　者：吕志强[1] 李海刚[2] 张惠忠[3] 范苗静[2] 沈溪明[2] 何欣欣[2] 

机构地区：[1]中山大学附属第二医院呼吸内科,广东广州510120 [2]中山大学附属第二医院病理科,广东广州510120 [3]中山大学附属第二医院心胸外科,广东广州510120

出　　处：《癌症》2008年第6期575-579,共5页Chinese Journal of Cancer

基　　金：广东省重点攻关项目(编号:2005B30601001);广州市科委科技攻关引导资助项目(02Z3-E0016)~~

摘　　要：背景与目的:人体各种组织都存在干细胞,肿瘤组织也存在肿瘤干细胞(tumor stem cell,TSC)。乳腺癌TSC已经被分离出来,其标记物也已被确定,但肺癌的TSC仍未被分离出来。本研究旨在探讨乳腺癌肿瘤干细胞标记物(CD44+ESA+CD24-Λow)在非小细胞肺癌(NSCLC)组织中的表达及其意义。方法:应用免疫组织化学法检测77例NSCLC组织中CD44、ESA与CD24的表达,分析其与患者吸烟、肿瘤的大小、癌的组织学类型、组织分化程度、淋巴结转移和预后的关系。结果:77例NSCLC组织中CD44、ESA与CD24的阳性率分别为63.6%、66.2%和7.8%。低分化及未分化组CD44的阳性率明显高于高分化组,高分化组ESA的阳性率明显高于中度分化组和低分化及未分化组;腺癌组ESA的阳性率明显高于鳞癌组(P<0.05)。CD44+ESA+CD24-Λow标记的阳性率为36.4%,与患者吸烟、肿瘤的大小、癌的组织学类型、组织分化程度、淋巴结转移和预后无关(P>0.05)。结论:乳腺癌肿瘤干细胞标记物(CD44+ESA+CD24-Λow)表达与NSCLC肿瘤的大小、癌的组织学类型、组织分化程度、淋巴结转移和预后等临床病理学指标无关。BACKGROUND ＆ OBJECTIVE： Stem cells are found in all human tissues, while tumor stem cells （TSCs） are also detected in tumors. TSC of breast cancer has been separated and its markers have been affirmed. However, TSC of lung carcinoma has not been separated yet. This study was to investigate the expression and significance of stem cell markers for breast cancer （CD44^＋ESA^＋CD24^∧ow） in non-small-cell lung carcinoma （NSCLC）. METHODS： Expressions of CD44, ESA and CD24 of tumor tissues in 77 cases of NSCLC patients were detected using immunohistochemistry. The correlations of the expression of the makers to tumor size, smoking, histological type, differentiation, lymphoid metastasis, and prognosis were analyzed. RESULTS： The expressive rates of CD44,ESA and CD24 were 63.6%, 66.2% and 7.8%, respectively in 77 NSCLC tissues. CD44-positive expression was significantly higher in poorly differentiated and undifferentiated group than in well differentiated group. ESA-positive expression was significantly higher in well differentiated group than in poorly differentiated and undifferentiated group. The ESA positivity was significantly higher in glandular carcinoma than in squamous carcinoma. The expressive rate of CD44^＋ESA^＋CD24^∧ow in 77 cases of NSCLC was 36.4%. No correlations were found in the expression of CD44^＋ESA^＋CD24^∧ow to smoking, tumor size, histological type, differentiation, lymphoid metastasis and prognosis （P〉0.05）. CONCLUSION： Expressions of stem cell markers for breast cancer （CD44^＋ESA^＋CD24^∧ow） are not associated with tumor size, histological type, differentiation, lymphoid metastasis and prognosis of NSCLC.

关 键 词：肺肿瘤 癌 非小细胞 免疫组织化学 肿瘤干细胞标记物 临床病理学 

分 类 号：R734.2[医药卫生—肿瘤]