表没食子儿茶素没食子酸酯对中波紫外线诱导人原代表皮黑素细胞光损伤干预作用的研究  

Intervention effects of epigallocatechin-3-gallate on photoproducts induced by UVB in primary human melanocyte

作　　者：高洁[1] 骆丹[1] 周炳荣[1] 徐晶[1] 林向飞[1] 朱文元[1] 

机构地区：[1]南京医科大学第一附属医院皮肤性病科,江苏南京210029

出　　处：《临床皮肤科杂志》2008年第6期345-347,共3页Journal of Clinical Dermatology

基　　金：国家自然科学基金资助项目(30371294)

摘　　要：目的:观察中波紫外线(UVB)诱导人原代表皮黑素细胞光产物的形成和清除情况,以及表没食子儿茶素没食子酸酯(EGCG)的干预作用。方法:选择10、20、40、80、100mg/L5种浓度EGCG作用于体外培养的人表皮黑素细胞72h,测定细胞增殖活性。采用免疫斑点印迹技术在30mJ/cm2UVB照射及EGCG干预下,分别取照射后0.5h和24h检测环丁烷嘧啶二聚体(CPDs)的产生和清除量。结果:低浓度(<20mg/L)EGCG能促进黑素细胞增殖。UVB照射后黑素细胞内光产物形成较快,但清除缓慢。EGCG对UVB诱导光产物的形成无明显影响,但能加速光产物的清除(P<0.05)。结论:EGCG能加速UVB照射后黑素细胞内光产物的清除。Objective： To investigate the production and clearance of photoproduets induced by UVB irradiation and the intervention effects of epigalloeateehin-3-gallate （EGCG） on human primary melanoeyte. Methods： The proliferation rates of melanoeytes were detected when cells had been incubated with 10, 20, 40, 80 and 100 mg/L EGCG for 72 h, respectively. The production and clearance of cyelobutane pyrimidine dimers （CPDs） were examined by immunodotblot assay at 0.5 and 24 hours after treatment by UVB and EGCG. Results： Low concentration of EGCG （〈20 mg/L） promoted the proliferation of cultured human melanoeytes. The production of UVB-indueed photoproduets was quick, while the clearance was slow. EGCG could facilitate the clearance of photoproduets, but had no effect on the CPDs formation. Conclusion： EGCG can facilitate the clearance of UVB-indueed photoproduets in cultured human melanoeytes.

关 键 词：表没食子儿茶素没食子酸酯 紫外线 中波 黑素细胞 环丁烷嘧啶二聚体 

分 类 号：R322.99[医药卫生—人体解剖和组织胚胎学]