枸骨叶总黄酮提取与测定  

Extraction and Determination of Total Flavonoids from Llex cornuta Lindl

作　　者：洪艳平[1] 上官新晨[1] 刘楠[1] 杨武英[1] 

机构地区：[1]江西农业大学食品科学与工程学院/植物资源开发与利用研究室,江西南昌330045

出　　处：《江西农业大学学报》2008年第3期529-533,共5页Acta Agriculturae Universitatis Jiangxiensis

基　　金：江西省科技厅农业攻关项目(200305001)

摘　　要：以芦丁为对照品,乙醇为浸提溶剂,利用紫外-分光光度法对枸骨叶总黄酮提取率进行测定。在单因素实验基础上采用L9(34)正交设计,考察了乙醇浓度、浸提时间、浸提温度、料液比等因素对枸骨叶总黄酮提取率的影响。研究结果表明,在浸提温度为80℃、料液比为1∶12的φ乙醇=20%中浸提3 h的条件下浸提效果最好,枸骨叶总黄酮提取率为3.975%,浸提级数为二级。The total flavonoids in Llex cornuta Lindl were determined through ultraviolet spectrophotometry with rutin as reference standard and ethanol as extracting liquid.The effective factors of ethanol concentration,temperature,treatment time and mass/volume ratio of solvent were studied through orthogonal tests[L9（3^4）]on the basis of single factor test.It was found that the extraction had the best result under the conditions：weigh ration-1∶12 of 20% ethanol to Llex cornuta Lindl,temperature-80 ℃,time-3 hours.The average extraction rate was 3.975% and the extraction series was 2.

关 键 词：枸骨叶 总黄酮 浸提 紫外-分光光度法 

分 类 号：S792.990.8[农业科学—林木遗传育种]