啤酒废水处理系统的高效菌株与微生物多样性  

High Efficient Bacteria and Microbial Diversity of Beer Wastewater Treatment Plants

作　　者：赵继红[1] 何淑英[2] 楼燕[1] 

机构地区：[1]河南工业大学化学与化工学院,郑州450001 [2]华东师范大学资源与环境科学学院,上海200062

出　　处：《河南师范大学学报（自然科学版）》2008年第3期73-75,共3页Journal of Henan Normal University(Natural Science Edition)

基　　金：河南省杰出青年科学基金(0512001500);郑州市科技攻关项目(052SGYS31193)

摘　　要：从啤酒废水处理系统SBR池取得的水样中分离筛选得到3株能高效降解啤酒废水COD的细菌,分别为12#、15#、23#.对3株高效菌株两两进行交互试验,考察混合菌株对废水的处理效果,并以高效菌株与活性污泥混合作用,23#与活性污泥交互作用明显,16 h后COD降解率达到75.13%;应用PCR-DGGE技术对啤酒废水生物处理系统的微生物多样性进行分析,对取自水解酸化池与SBR池中不同深度以及不同处理时段的水样,预处理后抽提基因组DNA,用细菌通用引物进行PCR扩增,PCR产物用变性梯度凝胶电泳进行分离,分析活性污泥样品中的微生物多样性.Three high efficient COD degradation bacteria are isolated from beer wastewater samples collected in SBR tank. Strains 12 # , 15 # , 23 # are inoculated in wastewater medium for interaction tests, and are mixed with activated sludge to identify the degradation ability for COD. The result shows that COD degradation rate of strain 23 # inoculated in wastewater medium is up to 75.13 % after 16 h. In addition, PCR-DGGE is used to study the microbial diversity in hydrolyze-acidification tank and SBR tank. Purified DNA fragments extracted from samples of different depths and disposal periods are amplified using universal bacterial primers and PCR products are analyzed with DGGE. DGGE fingerprints illustrate the microbial diversity of each activated sludge sample.

关 键 词：啤酒废水 高效菌株 PCR—DGGE 微生物多样性 

分 类 号：Q938[生物学—微生物学]