Sca-1^+Lewis肺癌细胞耐药实验研究  

Chemoresistance of Sca-1 positive cells in Lewis lung carcinoma cell line

作　　者：敖绪军[1] 安江宏[1] 钱莘[1] 卓文磊[1] 孙建国[1] 陈正堂[1] 

机构地区：[1]第三军医大学新桥医院全军肿瘤研究所,重庆400037

出　　处：《四川医学》2008年第5期498-500,共3页Sichuan Medical Journal

基　　金：国家科技计划863课题(2007AA02Z129);国家自然科学基金(30672076)

摘　　要：目的探讨小鼠Lewis肺癌(Lewis lung carcinoma,LLC)细胞株中Sca-1+LLC细胞的耐药特性及机制。方法将LLC细胞培养于含有不同浓度丝裂霉素、顺铂、健择、紫杉醇的培养液中,培养72h后分别检测各组Sca-1阳性细胞的比例。以Sca-1作为磁珠分选的表面标志,从LLC细胞中分选Sca-1+LLC细胞和Sca-1-LLC细胞,RT-PCR检测各组ABCG2mRNA表达水平。免疫荧光检测Sca-1+LLC细胞与SP细胞(Hoechst33342拒染)的关系。结果随着抗肿瘤药物浓度的升高,Sca-1阳性细胞的比例随之升高。RT-PCR检测表明Sca-1+LLC细胞表达更高水平的ABCG2mRNA,免疫荧光发现SP细胞只存在于Sca-1+LLC细胞。结论Sca-1+LLC细胞的耐药性较Sca-1-LLC细胞强,与Sca-1+LLC细胞高表达ABCG2有关。Objective To explored the chemoresistance mechanism of the Sea-1 positive cells in Lewis lung carcinoma（LLC） Cell Line.Methods LLC cells were planled in 6-well plates and different chemotherapeutic drugs were added in a concentration gradi- ent .After incubated for 72h, the percentage of Sca-1^＋ LLC cells were analyzed by fluorescence-actived cell sorting （FACS） . Then the Sca-1^＋LLC cells were sorted by magnetic activated cell sorting（MACS） ,RT-PCR was performed to analyze ABCG2 mRNA express in Sca-1^＋ and Sea-1^- populations,and immunofluorescence staining was used to detect the correlation between SP cell and ABCG2 expres- sion. Results The Sca-1^＋ LLC cells .were enriched with the increasing of chemotherapeutic drug＇s concentration, and the ABCG2 mRNA was expressed at high level in sorted Sca-1^＋ LLC cells. Immunohistoehemical analysis showed that SP cells were only found in the Sea-1^＋ LLC cells. Conclusion Sea-1^＋ LLC cells exhibite higher resistance to chemotherapeutic drugs than Sca-1^＋ LLC cells. The drug-resis-tance machanism may be relate to the over expression of ABCG2.

关 键 词：LEWIS肺癌 干细胞抗原-1 肿瘤干细胞 耐药性 

分 类 号：R734.2[医药卫生—肿瘤]