N-乙酰半胱氨酸对硫酸葡聚糖钠诱导的实验性肠炎的预防及治疗作用  

N-Acetyicysteine Alleviate the DSS Induced Experimental Colitis in Mice through Replenishment of Glutathione Level

作　　者：韩英[1] 张静[1] 纪欣[1] 王志红[1] 

机构地区：[1]北京军区总院消化内科,北京100700

出　　处：《中国医药导刊》2008年第1期130-133,共4页Chinese Journal of Medicinal Guide

基　　金：北京市自然科学基金项目(7042064)

摘　　要：目的:探讨还原型巨噬细胞诱导剂N-乙酰半胱氨酸(N-Acetylcysteine,NAC)对硫酸葡聚糖钠(Dextran Sodi- um Sulphate,DSS)诱导的小鼠实验性肠炎的预防及治疗作用。方法:建立DSS实验性小鼠肠炎模型,NAC及氧化型巨噬细胞诱导剂N,N’-联乙(?)-L-胱氨酸(N,N’-diacetyle-L-cystine,(NACOMe)2)在致模前后腹腔注射;检测腹腔巨噬细胞中还原型谷胱甘肽(GSH)与氧化型谷胱甘肽(GSSG)的变化;对结肠进行病理学评价,并检测结肠IL-4、IFN-g的表达。结果:NAC预处理组:结肠长度和病理学指标均优于其他预处理组,腹腔巨噬细胞内GSH及GSH/GSSG升高;结肠分泌的IL-4、IFN-γ明显降低。NAC治疗组:体重增加程度明显高于其它治疗组,结肠炎症及黏膜损伤较轻,腹腔巨噬细胞内GSSG明显减低,GSH和GSH/GSSG升高,病变结肠分泌的IL-4、IFN-γ明显降低。结论:还原型巨噬细胞诱导剂NAC可以通过诱导巨噬细胞内GSH产生增多从而减轻实验性肠炎的黏膜损伤及临床症状,对DSS诱导的实验性肠炎有一定的预防及治疗作用。Objective： To evaluate the preventive and therapeutic effect of N-Acetylcysteine（NAC）, an inductor of reductive macrophages （RMp） on DSS-induced experimental colitis of mice. Methods： The experimental colitis of mice was established by drinking with 5 % DSS. NAC or N, N ＇-diaeetyle -- L-cystine （（NACOMe） 2 ）, an inductor of oxidative macrophages,was administered before or after the model being established. The mice were killed at the 22nd day, the level of reductive glutathione （（;SH）,oxidative glutathione（GSSG） and the ratio of GSH/GSSG of macrophages in abdominal cavity was determined. The expression of IL-4 and IFN-g in colon was detected. Results： NAC pre-treated group scores better in terms of pathological parameters and the GSH ＆ GSH/GSSG are significantly higher, the IL-4 produced by colonic mucosa is significantly lower compared with the other pre-treated groups. After the colitis model was established, the colonic inflammation in NAC treated group was significantly improved and the GSSG within maerophage was significantly reduced paralleling with the increase of GSH ＆ GSH/GSSG, meanwhile IFN-γ IL-4 remarkably de- creased compared with other groups. Conclusion： NAC, an inductor of reductive macrophages （RMp） can alleviate experimental colitis in mice through replenishment of GSH and might be used in prevention and treatment of DSS-indueed experimental colitis.

关 键 词：N-乙酰半胱氨酸 N N'-联乙醯-L-胱氨酸 硫酸葡聚糖钠 实验性肠炎 巨噬细胞 谷胱甘肽 

分 类 号：R574[医药卫生—消化系统]