MTHFR基因多态性和酗酒与缺血性脑卒中的相关性  

Correlation of polymorphism of mehylenetrahydrofolate reductase gene and alcohol abuse with ischemic stroke

作　　者：梁银杏[1] 吴婉玲[1] 姚晓黎[1] 李才明[2] 莫照龙[3] 张为西[1] 苏全喜[4] 卢锡林[1] 

机构地区：[1]中山大学附属第一医院神经科,广东广州510080 [2]惠州市中心人民医院,广东惠州516001 [3]海南省农垦总局医院,海南海口570311 [4]广东药学院第一附属医院神经科,广东广州510080

出　　处：《中国热带医学》2008年第6期903-905,共3页China Tropical Medicine

基　　金：广东省博士启动基金资助项目(编号:5300783;04300353);广东省科技计划项目(编号:2006B36003003)

摘　　要：目的探讨N5,N10-亚甲基四氢叶酸还原酶(MTHFR)基因多态性和酗酒与缺血性脑卒中(IS)发病风险的关系。方法应用聚合酶链反应(PCR)和变性高效液相色谱(DHPLC)技术筛查454例IS患者(病例组)和334例非IS患者(对照组)的MTHFR基因的多态分布,采用非条件Logistic回归模型分析基因型、酗酒情况与缺血性脑卒中发病风险的关系。结果酗酒群体的T等位基因的频率显著性升高(P<0.05),患缺血性脑卒中的相对危险度为2.633;而携带有CC基因型则为0.360。相反,非酗酒群体的MTHFR基因的各基因型和等位基因频率的分布与对照组相比,均无显著性差异(P>0.10)。结论携带T等位基因的酗酒群体容易患缺血性脑卒中,但携带C等位基因的酗酒群体不容易患缺血性脑卒中,MTHFR基因与酗酒在缺血性脑卒中的发病过程中存在协同作用。Objective To investigate correlation of ischemic stroke（IS） with methylenetrahydrofolate reductas（MTHFR） gene and alcohol abuse. Methods Four hundred and fifty -four patients and 334 controls were recruited in this observation. CT polymorphisms of MTHFR gene were detected by polymerase chain reaction （PCR） and denaturing high performance liquid chromatogram（DHPLC）. The correlation of ischemic stroke with methylenetrahydrofolate reductas gene and alcohol abuse were analyzed by odds ratios （ORs） and 95% confidence intervals （95% CIs）. The value of 95% CIs were calculated by unconditional logistic regression. Results The frequencies of T alleles in ischemic stroke patients with alcohol abuse were significantly higher than those of the controls with OR of 2.63, but the OR of CC genotypes ischemic stroke patients with alcohol abuse was 0.360, individually. On the other hand, the OR of all genotypes and alleles in ischemic stroke patients with non - alcohol abuse patients were not significantly different than those in controls. Conclusion The alcohol abusers with T alleles are likely to suffer from ischemic stroke, but those with C alleles are not likely to suffer from ischemic stroke, and there are interactions between alcohol abuse and MTHFR gene in the pathogenesis of ischemic stroke.

关 键 词：缺血性脑卒中 N^5 N^10-亚甲基四氢叶酸还原酶 基因 酗酒 

分 类 号：R255.2[医药卫生—中医内科学]