理想肌桥桥接神经缺损构型个性化差异对神经再生的影响  

Effects of individual differences of constructed muscle bridge on neural regeneration

作　　者：张勇[1] 杨晓霞[1] 苏秋香[1] 张庭深[1] 

机构地区：[1]沈阳医学院组织胚胎学教研室,辽宁省沈阳市110034

出　　处：《中国组织工程研究与临床康复》2008年第20期3810-3813,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景：发展修复周围神经系统的技术已成为生物学家、生化工程师及材料学家多学科共同参与的重要研究课题。目的：实验拟观察理想肌桥桥接神经缺损构型个性化差异对神经再生的影响。设计、时间及地点：对比观察，于2004-06／2005—09在沈阳医学院完成。材料：10只普通家犬，体质量20～30k。方法：在静脉麻醉下，将犬右侧缝匠肌尾侧部和指浅屈肌中段肌条分别切成两段，一段置于体视镜下剥离观察；另一段常规苏木精-伊红染色后用图像分析其远、近端横断面的肌纤维截面积。取两种肌肉肌条分别缝接于自体左、右肢股神经缺损2cm处，常规锇酸染色后测定有髓神经纤维的数量。主要观察指标：①肌桥的形态差异，肌条远、近端横断面的肌纤维截面积。②桥接左、右肢股神经缺损处有髓神经纤维的数量。结果：①缝匠肌肌条内的肌纤维与肌条长轴基本平行；指浅屈肌肌条内的肌纤维走向与肌条长轴夹角较大。缝匠肌肌条内80％的肌纤维与肌条等长：指浅屈肌肌条内肌纤维则均短于肌条长度。指浅屈肌肌条内的肌纤维较粗，缝匠肌肌条内的肌纤维较细，两种肌桥内肌纤维的截面积有较大差异（P〈0．01）。②在锇酸染色标本上，有髓神经纤维的髓鞘呈粗细不等的黑色圆圈状。用缝匠肌尾侧部桥接的左侧股神经远段的有髓神经纤维明显比用指浅屈肌桥接的右侧股神经远段的有髓神经纤维多。结论：由相对长度较大的肌纤维为主要构成的肌桥桥按缺损神经的再生效果较好。BACKGROUND： Studies on the reparation of peripheral nervous system are not only a key focus in medical research, but also a major topic for biologists, biochemical engineers and materialogy specialists. OBJECTIVE： To investigate the individual difference of ideal muscle bridging neurologic defect configuration on nerve regeneration. DESIGN, TIME AND SETTING： The contxol experiment was performed at the Shenyang Medical College from June 2004 to September 2005. MATERIALS： Ten common dogs weighing 20-30 kg were used in this study. METHODS： After intravenous anesthesia, muscle strips in the caudal right sartorius and middle musculus flexor digitorum superficialis of dogs were sliced into two segments. One was observed by dissection under a stereoscope. Another received Haematoxylin ＆ Eosin （HE） Staining and txansverse area of muscle fiber of distal and proximal ends was analyzed by image analysis. The same muscle strip was sutured at 2 cm neurologic defect region of autologous left and right limbs. Number of medullated nerve fibers was measured after osmic acid staining. MAIN OUTCOME MEASURES： Morphological difference of muscle bridge, txansverse area of muscle fiber of distal and proximal ends, and number of medullated nerve fibers at bridging femoral nerve defect of left and right limbs. RESULTS： The muscle fiber in sartorius paralleled the longitudinal axis of muscle strip. The angle between muscle fiber in musculus flexor digitorum superficialis and the longitudinal axis of muscle strip was big. 80% muscle fiber in sartorius was equa I to the length of the muscle bridge. Muscle fiber in the musculus flexor digitorum superficialis was shorter than the length of muscle strip. Muscle fiber in the musculus flexor digitorum superficialis was thick, whereas muscle fiber in sartorius was thin. There were significantly differences in txansverse area of muscle fiber in the two kinds of muscle bridges （P 〈 0.01）. Medullary sheath of medullated nerve fibers showed black circle-shape in di

关 键 词：肌桥 肌纤维长度 肌纤维横截面积 神经再生 

分 类 号：R741[医药卫生—神经病学与精神病学]