短程高剂量重组人粒细胞-集落刺激因子对小鼠外周血造血干细胞的动员作用  

Mobilization of peripheral blood stem cells in mice with short-duration and high-dose recombinant human granulocyte colony-stimulating factor

作　　者：闫金松[1] 童金生[1] 宋振岚[1] 

机构地区：[1]大连医科大学附属第二医院血液风湿科,辽宁省大连市116027

出　　处：《中国组织工程研究与临床康复》2008年第21期4089-4092,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:外周血造血干细胞用于损伤组织的修复及再生需要快速有效的动员方法。常规的动员方案多采用重组人粒细胞-集落刺激因子或大剂量化疗联合重组人粒细胞-集落刺激因子,该方案耗时长、程序复杂。目的:对常规的外周血造血干细胞动员方案进行改进,观察短程高剂量重组人粒细胞-集落刺激因子动员KM小鼠外周血造血干细胞的效果。设计、时间及地点:于2006-09/2007-03在大连医科大学附属第二医院血液科实验室开展的随机对照动物实验。材料:雄性8周龄KM小鼠24只,以给药方式分为3组:常规给药组、短程高剂量给药组、生理盐水对照组。方法:常规给药组,皮下注射重组人粒细胞-集落刺激因子,0.2μg/次,2次/d,共6d;短程高剂量给药组,前4d注射等量生理盐水,后2d皮下注射重组人粒细胞-集落刺激因子,2.5μg/次,2次/d;生理盐水对照组,每日注射等量生理盐水,共6d。主要观察指标:各组小鼠外周血白细胞计数、粒-巨核细胞集落数。结果:常规剂量组及短程大剂量组小鼠外周血白细胞迅速增高,对照组无明显变化。粒-巨核细胞集落培养7d后,常规剂量组和短程高剂量组的粒-巨核细胞集落计数较对照组显著升高达50倍以上(P<0.01),常规剂量组和短程高剂量组两组间比较差异无显著性意义(P>0.05)。结论:短程高剂量重组人粒细胞-集落刺激因子动员小鼠外周血造血干细胞,有着良好的可行性及有效性。BACKGROUND： The prompt and efficient peripheral blood stem cell （PBSC） mobilization is necessary for repairing and regenerating injured tissues. Recombinant human granulocyte colony-stimulating factor （rhG-CSF） or its combination with a large dose of chemotherapy is usually utilized for PBSC mobilization, but the regimen requires a long time and a complex procedure. OBJECTIVE： To modify the common PBSC mobilization, and investigate mobilization effect of PBSC in KM mice with short-duration and high-dose rhG-CSF. DESIGN, TIME AND SETTING： A randomized controlled animal trial was carried out in the laboratory of Hematology, the Second Affiliated Hospital to Dalian Medical University from September 2006 to March 2007. MATERIALS： Twenty-four KM male mice of 8 months old were divided into 3 groups according to the administration： conventional regimen group, short-duration and high-dose group, and saline control group. METHODS： Mice in the conventional regimen group were injected subcutaneously with rhG-CSF （0.2 μ g one time, twice a day for 6 days）. Mice in the short-duration and high-dose group were injected with equal volume of normal saline at first 4 days, and then with rhG-CSF （2.5 μ g one time, twice a day for 2 days）. Mice in the saline control group were injected subcutaneously with equal volume of normal saline for 6 days. MAIN OUTCOME MEASURES： Peripheral blood white blood cell （WBC） and colony-forming unit-granulocyte macrophage in KM mice were counted. RESULTS： WBC in the peripheral blood of KM mice increased rapidly in conventional regimen group and short-duration and high-dose group. No obvious change of WBC was observed in the control group. The number of colony-forming unit-granulocyte macrophage in conventional regimen group and short-duration and high-dose group was significantly elevated over 50 folds than control group （P 〈 0.01）, and there was no statistical significance between conventional regimen group and short-duration and high-dose grou

关 键 词：外周血造血干细胞 动员 粒细胞集落刺激因子 组织损伤 小鼠 

分 类 号：R394.2[医药卫生—医学遗传学]