靶向IGF-ⅠR的RNA干扰技术抑制胃癌细胞增殖的研究  

Study on the inhibition of proliferation on gastric cancer cells by RNA interference targeting to IGF-ⅠR

作　　者：牛海静[1] 方维丽[1] 王邦茂[1] 曹晓沧[1] 丁娟娟[1] 

机构地区：[1]天津医科大学总医院消化内科,天津300052

出　　处：《中国实验诊断学》2008年第5期583-586,共4页Chinese Journal of Laboratory Diagnosis

基　　金：天津市科学技术委员会资助项目(05KYZ79)

摘　　要：目的观察特异的小干扰RNAs(small interfering RNAs,siRNAs)对胃癌细胞胰岛素样生长因子-Ⅰ受体(In-sulin-like growth factor-Ⅰ receptor,IGF-ⅠR)基因表达的抑制作用,研究其对肿瘤增殖的影响。方法设计并体外合成2条靶向IGF-ⅠR的siRNAs,脂质体法瞬时转染MGC803细胞,转染前后均使用WesternBlot法检测IGF-ⅠR的表达水平,MTT法检测细胞增殖,细胞计数并描记生长曲线。结果MGC803细胞中有IGF-ⅠR的强烈表达。转染后48h,干扰组(siRNA2-L组、siRNA2-H组、siRNA1组)IGF-ⅠR表达抑制率分别为64.41%±4.11%、74.14%±6.15%、89.8%±4.10%;siRNA1组转染后第2-5天细胞增殖逐渐减少(分别达49.9%±1.2%、45.9%±4.4%、39.1%±5.1%、29%±4.0%);同期细胞计数分别为对照组的65.58%±4.89%、55.59%±0.82%、44.18%±3.17%、21.15%±1.1%。结论特异的siRNAs可显著抑制胃癌细胞中的IGF-ⅠR基因的表达从而显著抑制细胞的增殖。Objective To observe the expression of IGF- I R on human gastric cancer MGC803 cells inhibited by special siR- NAs,and investigate the following effects of the cell proliferation. Methods Two different siRNAs targeted to IGF- I R were de- signed,synthesized and transfected into MGC803 cells,the expression of IGF- I R were detected by Western Blot before and after the transfection,then the cell proliferation were examined by MTI＇,the growth curve was obtained.Results Intensive expression of IGF- I R was shown in MGC803 cells.The inhibition ratio of IGF- I R protein of interfering groups（siRNA2-1ow dose group, siRNA2-high dose group, siRNA1 group） were 64.41% ± 4.11% ,74.14% ± 6.15% ,89.8% ± 4.10% respectively 48 hours after transfeetion; The cell number gradually decreased by 49.9% ± 1.2% ,45.9% ± 4.4% ,39.1% ± 5.1% ,29% ± 4.0% at the same time. Conduon Special siRNAs can remarkably inhibit the expression of IGF- I R in gastric cancer cells, the cell proliferation was decreased greatly.

关 键 词：小干扰RNA IGF-ⅠR 胃癌 

分 类 号：R735.2[医药卫生—肿瘤]