电针膀胱经五脏俞对格林-巴利综合征家兔协同刺激分子影响的研究  

The influence on costimulatory molecules expression by electroacupuncture at Shu-points of the Five Zang-organ for the acute allergy neuritis in domestic rabbit's

作　　者：王洪峰[1] 东贵荣[2] 

机构地区：[1]长春中医药大学,长春130117 [2]上海中医药大学,上海201203

出　　处：《中国免疫学杂志》2008年第6期530-532,537,共4页Chinese Journal of Immunology

基　　金：国家中医药管理局科研课题(No.04-05JP17)

摘　　要：目的:通过观察电针膀胱经五脏俞对格林-巴利综合征(GBS)家兔坐骨神经中协同刺激分子的影响,探讨电针膀胱经五脏俞治疗GBS的作用机制。方法:采用国际公认的P2免疫动物模型(EAN),酶联免疫吸附测定法(ELISA)观察电针膀胱经五脏俞对GBS家兔坐骨神经中协同刺激分子CD28、CTLA-4、B7-1、B7-2的蛋白表达水平。结果:经过治疗后CD28、B7-1表达水平明显降低(P<0.05),CTLA-4、B7-2表达水平明显升高(P<0.05)。结论:电针膀胱经五脏俞通过使协同刺激分子CD28、B7-1蛋白表达水平明显降低,CTLA-4、B7-2蛋白表达水平的明显升高,参与EAN的免疫调节。Objective：To observe the influence of electroacupuncture at Shu-points of the Five Zang-organ in domestic rabbits with acute allergy neuritis, a classical animal model, and to explore the immunoregulatory mechanism responsible for the regimen by detecting expression of the eostimulatory molecules in sciatic nerve. Methods： ELISA was used to examine proteins of CD28, CTLA-4, B7-1, B7-2 in sciatic nerve of P2 immunized EAN domestic rabbits. Results： It was showed that the expression of CTLA-4 and B7-2 was obviously up-regulated（P 〈 0.05） ,whereas the expression of CD28, B7-1 was obviously down-regulated（P 〈 0.05）. Conclusion： Up-regulation of CD28 and B7-1, and down-regulation of CTLA-4 and B7-2 may participate in the modification of EAN by electroacupuneture at Shu-points of the Five Zang-organ.

关 键 词：电针 五脏俞 格林-巴利综合征 协同刺激分子 

分 类 号：R745.43[医药卫生—神经病学与精神病学]