中国HIV-1 B’/C亚型感染者对自身病毒中和作用与疾病进展关系的研究  

Study on the relationships between neutralizing antibody response and disease progression of HIV type 1 B'/C infected individuals in China

作　　者：包名家[1] 耿文清[2] 崔华露[2] 张晓丽[3] 徐东兵[2] 年华[2] 赵敏[2] 王亚婷[2] 姜拥军[2] 王亚男[2] 韩晓旭[2] 张旻[2] 张子宁[2] 尚红[2] 

机构地区：[1]黑龙江省佳木斯市疾病预防控制中心,佳木斯154002 [2]中国医科大学附属第一医院卫生部艾滋病免疫学重点实验室,沈阳110001 [3]黑龙江省佳木斯大学附属第一医院检验科,佳木斯154007

出　　处：《中国免疫学杂志》2008年第6期558-562,共5页Chinese Journal of Immunology

基　　金：“十五”国家科技攻关课题项目(2004BA719A12);辽宁省科技攻关课题(2005225001);辽宁省教育厅高等学校创新团队项目(2006T134);国家重点基础研究发展计划(973计划)(2006CB504206)资助

摘　　要：目的:探讨中国HIV-1 B’/C亚型感染者对自身病毒中和作用与疾病进展的关系。方法:将24例HIV-1 B’/C感染者自身原代病毒与同期和6个月自身血浆作用后,感染正常PBMC,培养7天测定p24抗原浓度,以正常人血浆加病毒悬液为对照。以抑制50%对照孔p24浓度的血浆最高稀释倍数的倒数计算中和抗体滴度,中和抗体滴度≥8倍为具有中和作用。结果:在同期血浆中和自身病毒试验中,3例缓慢进展者(SP)均具有中和作用,HIV组仅4例(4/21)具有中和作用,SP组中和抗体滴度明显高于HIV组。在6个月血浆中和自身病毒试验中,SP组中和抗体滴度明显增加,HIV组12例具有中和作用,SP组中和抗体滴度明显高于HIV组。中和抗体滴度与病毒载量呈明显的负相关。结论:疾病缓慢进展的HIV-1 B’/C亚型感染者对自身病毒中和作用明显高于HIV组,提示中和抗体在延缓疾病进程中发挥重要作用。Objective：To study the relationships between neutralizing antibody response against autologous virus and disease progression of HIV-1 B＇/C infected individuals in China. Methods： Twenty-four primary HIV-1 isolates were incubated with autologous plasma collected either freshly or at approximately six months intervals thereafter. Normal human peripheral blood mononuclear cells were incubated with the virus-serum mixtures for 7 days and then the production of p24 antigen was measured. The neutralizing titer of a parficular plasma and virus was defined as the reciprocal of the highest dilution giving a 50% reduction in p24 Ag compared with NHP control wells. More than 1：8 were considered significant and were scored as positive. Results： In neutralizing antibody （Nabs） response against contemporaneous virus, Nabs were produced in all slow pmgressors （SP） individuals, while only four in 21 of HIV group had. There was statistically significance of the neutralizing antibody titers between SP and HIV. When plasma samples of six months later were tested for their ability to neutralize autologous virus, all of SPs had higher neutralizing antibody titers and the tilers of neutralizing antibody in HIV group had increased in different rate. Among the twenty- one individuals of HIV group, 12 of these individuals had neutralizing antibody response against autologous virus and other 9 of these individuals had not. NAb titers of SP in six months later plasma were higher than those of HIV. There was a negative correlation between the generation of the neutralizing titer against autologous vires and the plasma HIV RNA level in HIV- 1 B＇/C infected individuals （ including SP, HIV）. Conclusion： Neutralizing antibody against autologous viruses in HIV-1 B＇/C infected SP is higher than those of HIV group,suggesting that neutralizing antibodies play a vital role in delaying disease progression in these individuals.

关 键 词：HIV-1 B’/C亚型 中和抗体 疾病进展 

分 类 号：R512.91[医药卫生—内科学]