褪黑素对动脉粥样硬化模型兔动脉肌球蛋白轻链激酶表达及活性的影响  

Effect of melatonin on expression and activity of myosin light chain kinase in the artery wall of atherosclerotic rabbits

作　　者：朱华庆[1] 程筱雯[1] 江志奎[1] 肖林林[1] 左莉[1] 胡若磊[1] 张素梅[1] 周青[1] 桂淑玉[1] 汪渊[1] 

机构地区：[1]安徽医科大学分子生物学实验室和生物化学教研室,安徽省和省部共建重要遗传病基因资源利用重点实验室,安徽合肥230032

出　　处：《中国药理学通报》2008年第5期606-610,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30570750);安徽省自然科学基金(No070413079);安徽省教育厅基金资助项目(NoKJ2007B275)

摘　　要：目的探讨褪黑素(melatonin,MLT)对动脉粥样硬化模型兔动脉肌球蛋白轻链激酶(MLCK)表达与活性的影响。方法复制动脉粥样硬化兔模型,采用免疫组化和Western blot分析主动脉MLCK蛋白表达,γ-32P-ATP参入法检测MLCK活性。结果成功建立动脉粥样硬化兔模型,高脂喂食12wk,兔主动脉MLCK蛋白表达水平增加,活性上升,经MLT治疗后动脉粥样硬化斑块减少,MLCK蛋白表达下降,活性下降。结论动脉粥样硬化的形成与MLCK蛋白表达与活性升高有关,MLT能降低动脉MLCK蛋白表达和活性。Aim To study the effect of melatonin on expression and activity of myosin light chain kinase in the artery wall of atherosclerotic rabbits. Methods The rabbit model of atherosclerosis was induced by a high-cholesterol diet. The blood lipid levels were assayed in the serum of each group. MLCK expression was detected by Western blot and immunohistochemical method. MLCK activity was measured by γ-32p-ATP incorporation into myosin light chain. Results The ath- erosclerosis model was established successfully. The levels of lipids decreased after MLT treatment. After fed with cholesterol ＇for twelve weeks, the expression and activity of MLCK in the artery of atherosclerotic rabbits increased markedly, whereas there was no obvi- ous difference in expression of MLCK in the artery of atherosclerotic rabbits fed with cholesterol and melato- nin for twelve weeks compared with that of control. Conclusions It was suggest that high expression and activity of MLCK in the artery might be closely correla- ted with the development of atherosclerosis. Melatonin played an important role in inhibiting the development of atherosclerosis by decreasing the expression and ac- tivity of MLCK.

关 键 词：褪黑素 动脉粥样硬化 肌球蛋白轻链激酶 Western BLOT 

分 类 号：R-332[医药卫生] R322.121