丹参酮-ⅡA磺酸钠对慢性低氧性肺动脉高压大鼠的治疗作用  

The therapeutical effect of sodium tanshinone ⅡA sulphonate on hypoxic pulmonary hypertension rats

作　　者：刘曼玲[1] 李志超[1] 董明清[1] 王晓斌[1] 许敏[1] 刘毅[1] 董海莹[1] 黄玉芳[1] 牛雯[1] 

机构地区：[1]第四军医大学病理生理学教研室,陕西西安710032

出　　处：《中国药理学通报》2008年第6期723-726,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30770925;30700965;30400155)

摘　　要：目的探讨丹参酮-ⅡA磺酸钠(STS)对大鼠慢性低氧性肺动脉高压(HPH)的治疗作用。方法采用间断性低压低氧法建立SD大鼠慢性HPH模型。将大鼠随机分成:常氧组、常氧+STS组、低氧组和低氧+STS组。造模后测定平均左颈总动脉压(mean carotid arterial pressure,mCAP)、右心室收缩压峰值(right ventricular peak systolic pressure,RVP-SP)、右心室肥厚指数〔RV/(LV+S)%〕、肺小动脉管壁厚度占外径的百分比(WT%)及管壁面积占血管总面积的百分比(WA%)和肺组织匀浆中SOD与MDA含量。结果低氧+STS组大鼠的RVPSP与低氧组相比明显降低(P<0.01),而各组大鼠的mCAP无差别。HE染色结果显示常氧组与常氧+STS组肺组织结构均正常,而低氧组可见肺泡腔水肿,点、片状渗出、出血,肺小动脉管壁和平滑肌层明显增厚,管腔狭窄;低氧+STS组较低氧组明显减轻,平滑肌层呈不规则间断性肥厚,管腔部分狭窄。低氧+STS组大鼠右心室肥厚指数〔RV/(LV+S)%〕、肺小动脉WA%和WT%值、肺组织MDA含量也明显低于低氧组(P<0.01),SOD含量高于低氧组(P<0.01);常氧组与常氧+STS组则无差别。结论STS能够抑制大鼠慢性HPH及其所引起的心、肺血管的病理变化,这种作用可能与其降低氧化应激有关。Aim To observe the role of sodium tanshinone IIA sulphonate （STS） in the prevention of hypoxic pulmonary hypertension in rats. Methods The animal model of hypoxic pulmonary hypertension was established by exposing the rats to hypobaric hypoxia environment for 3 weeks. Thirty-four male SD rats were randomly divided into four groups：normoxia group, normoxia ＋ STS group,hypoxia group and hypoxia ＋ STS group. The mean carotid arterial pressure （mCAP） and the right ventricular peak systolic pressure （RVPSP） were examined by catheterization, and the RV/（ LV ＋ S） % was calculated. The lung specimens were exam- ined by light microscope to study WA%, WT% and morphological analysis. Meanwhile, SOD and MDA in lung homogenate were measured. Results The RVPSP significantly decreased in hypoxia ＋ STS group compared with that in hypoxia group （ P 〈 O. O1 ）. At the same time, the mCAP was of no difference in all groups. Histological study showed that there was con- gestion, edema, the sequestration of inflammatory cells and the small pulmonary artery vessel wall was thick- ened and the vessel lumina was deflated in lung tissues in hypoxia group, which were significantly alleviated by STS treatment. The RV/（ LV ＋ S ） %, the WA%, the WT%, and the content of MDA in hypoxia group in- creased,but the content of SOD in hypoxia group decreased. STS treatment could inhibit all those increased parameters （ P 〈 0. 01 ）, and upgrade the content of SOD （ P 〈 0. 01 ）. Conclusions STS could play an important role in treatment of hypoxic pulmonary hypertension of rats, and the mechanism of protection was correlated with inhibition of oxidative stress.

关 键 词：肺动脉高压 丹参酮ⅡA磺酸钠 超氧化物歧化酶 丙二醛 

分 类 号：R-332[医药卫生] R284.1