莫达非尼对大鼠海马神经元IGABA的抑制作用机制  

Pharmacological properties of modafinil in rat hippocampus pyramidal neurons

作　　者：王元银[1] 黄擎[1] 唐慧[1] 王烈成[2] 

机构地区：[1]安徽医科大学口腔医学院,安徽合肥230032 [2]安徽医科大学基础医学院,安徽合肥230032

出　　处：《中国药理学通报》2008年第6期727-730,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30670694);安徽省教育厅自然科学研究基金资助项目(NoNSFA2006KJ361B)

摘　　要：目的研究新型促醒药莫达非尼对培养的大鼠海马神经元GABAA受体介导的电流(IGABA)的影响。方法在培养的海马锥体神经元上,进行全细胞膜片钳记录。结果GABA引起电流能被GABAA受体的竞争性阻断剂荷包牡丹碱阻断。单独给予莫达非尼时,明显地抑制了内向电流IGABA。而格列本脲预处理海马神经元后,再单独给予莫达非尼,发现莫达非尼对于IGABA的抑制作用被逆转。结论新型促醒药莫达非尼不同于传统的兴奋剂,莫达非尼对于神经元的保护作用可能与ATP敏感性钾离子通道的开放有关。Aim To study the effects of a novel psychoactive drug, modafinil, on the GABAA receptor-induced current in rat hippocampus pyramidal neurons. Methods Whole-cell patch-clamp recordings were applied in cultured hippocampus pyramidal neurons. Results The GABA-activated currents were blocked by bicuculline, a competitive antagonist of GABAA receptor. Modafinil could partly inhibit IGABA Pre-treating hippocampus neurons with glibenclamide for 30 min, modafinil-induced inhibition on IGABA was removed. Conclusion Modafinil is different from other psychostimulants. The protection of modafinil may be via modulating ATP sensitive potassium channel activa- tion.

关 键 词：莫达非尼 GABA ATP敏感性钾离子通道 海马神经元 膜片钳 

分 类 号：R-332[医药卫生] R338