AY245441基因在小鼠腭突发育和腭裂形成中的动态表达  

Temporal and Spatial Expression of AY245441 gene during Development of Palate Shelf and Formation of Cleft Palate in Mice

作　　者：朱江波[1] 万旭英[1] 朱玉平[1] 朱勇飞[2] 马玺里[1] 张天宝[1] 

机构地区：[1]第二军医大学卫生毒理学教研室,上海200433 [2]杭州师范大学医药卫生管理学院,浙江杭州310018

出　　处：《癌变．畸变．突变》2008年第3期178-181,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金资助课题(30400360;30070662)

摘　　要：背景与目的：观察AY245441基因在正常腭组织和全反式视黄酸诱导的小鼠腭裂形成过程中的表达变化，探讨AY245441基因的表达在腭突正常发育和腭裂发生中的作用。材料与方法：利用Real-timePCR和原位杂交方法动态检测正常腭组织和全反式视黄酸诱导的腭裂组织中AY245441基因的表达情况。结果：Real-timePCR结果表明，AY245441基因在GD11～16正常腭突和腭裂组织中均有表达，在正常腭中GD11，GD13和GD14时AY245441表达丰度最高，与其它胚龄相比较差异具有统计学意义（P〈0．05）；腭裂组中AY245441的表达丰度明显低于正常腭（P〈0．05），而在腭裂组织中各胚龄AY245441的表达丰度差异无统计学意义（P〉0．05）。原位杂交结果显示，AY245441基因在GD13正常腭板前部的上皮细胞，GD14腭板前部上皮细胞和间充质细胞中均有表达；在GD14正常腭板后部上皮细胞中有少许表达，但间充质细胞中无表达。在GD13腭裂前部上皮细胞中表达量明显下降，在GD14腭裂前部上皮细胞和间充质细胞中表达受到明显抑制，几乎未检出阳性信号；在GD14腭裂后部上皮细胞和间充质细胞中均无表达。结论：AY245441基因在小鼠腭突组织的生长发育中行使重要功能，与腭裂的发生有密切的关系。[ABSTRACT] BACKGROUND AND AIM： To evaluate the expression of the AY245441 gene in normal embyonic palatine process and the cleft palate of mice induced by retinoic acid and to explore the effects between the AY245441 gene function and the formation of cleft palate . MATERIALS AND METHODS： The expression of the AY245441 gene in embyonic palatine process tissues in the experimental group induced by retinoic acid and control groups were assessed by Real-time PCR from GDll-GD16 and in situ hybridization on GD13 and GD14. RKSULTS： AY245441 was expressed in all normal and cleft palates at GDll-GD16. To the normal palates, the highest expressions were in GDll, GD13 and GD14, showing significant differences to the samples （P 〈 0.05） taken at other gestational dates. The expression abundance of AY245441 in normal palates was stronger than that of cleft palates during GDll- GD16（P〈0.05） AY245441 expression was detected in beth the epithelium of the anterior region of normal developing palatal shelves at GD13 , in beth the epithelium and mesenchyme of the anterior region of normal developing palatal shelves at GD14. AY245441 gene expression was detected in the epithelium , but was suppressed in the anterior region of cleft palates at GD13. AY245441 gene expression was absent in beth the epithelium and mesenchyme of the anterior region of cleft palatal at GD14. AY245441 was absent in beth the epithelium and mesenchyme of normal posterior palate and Cleft palate at GD14. CONCLUSION： The AY245441 gene played an important role in the development of palate in mice .The suppressed expression of the AY245441 gene was related to the occurrence of cleft palate.

关 键 词：AY245441基因 腭裂 REAL-TIME PCR 原位杂交 

分 类 号：R782.22[医药卫生—口腔医学]