TiO_2纳米光催化剂薄膜对人工晶状体表面修饰作用及其意义  

TiO_2 nanometer thin film on surface modificaiton of IOL and its significance

作　　者：单玫[1] 翁景宁[1] 

机构地区：[1]福建医科大学附属协和医院眼科,福建省福州市350001

出　　处：《眼科新进展》2008年第6期407-411,共5页Recent Advances in Ophthalmology

基　　金：福建省自然科学基金资助(编号:C0510017);福建省联合攻关基金资助(编号:WKJ2005-2-003)~~

摘　　要：目的研究经光激发后纳米TiO2光催化剂薄膜对体外培养的牛晶状体上皮细胞(lens epithelial cells,LEC)的杀伤效应。方法采用溶胶-凝胶法在玻片表面制备TiO2光催化剂薄膜。体外培养牛LEC爬片生长于此薄膜上。设未经纳米TiO2光催化剂薄膜修饰的玻片为对照。UVA分组激发玻片0～50min后,观察细胞形态,MTT比色法测定各组细胞存活率并比较。用HE染色、AO-EB染色观察牛LEC死亡现象。结果经UVA激发的纳米TiO2光催化剂薄膜对牛LEC有明显杀伤作用,光镜下见实验组激发20min以上细胞表面有大量泡状突起,大部分细胞收缩变圆;对照组细胞未见明显改变。实验组与对照组细胞存活率有统计学意义,且随激发时间延长,TiO2薄膜对牛LEC的杀伤效应更加显著。HE染色可见实验组激发20min以上,细胞收缩变圆,细胞间隙增大,核固缩明显,未见核仁,部分胞膜破裂,细胞崩解为碎片;对照组细胞形态大致正常。AO-EB染色发现受激发40min,实验组大量牛LEC坏死。结论受激发的纳米TiO2光催化剂薄膜对牛LEC有明显杀伤作用。推断细胞死亡机制可能是TiO2受激发后产生超氧自由基等活性氧物质,在细胞膜表面产生氧化还原反应所致。Objective To investigate the killing effect ofphotoexcited TiO2 nanometer film on bovine lens epithelial cells （LEC）. Methods TiO2 nanometer films were prepared via the sol-gel method on glass plates,which were used to culture borine LEC, and naked plates were as control. Under UVA irradiation for 50 minutes, the morphological changes of cells were observed, and the cell viability was tested by MTT chromatometry; The expiration of cells were observed with HE and AO-EB assay. Results The photoexcited TiO2 film showed significant killing effect on bovine LEC. Exposing of cells to UVA more than 20 minutes resulted in the membrane blebbing and shrinking on TiO2 film. No significant change was found in control group. There was statistical difference on cell vitality between control group and experimental group. With the exposure time increased,the killing effect of TiO2 film on bovine LEC became more significant. After UVA irradiation for 20 minutes, the cell membrane rupture,karyopyknosis,nucleolus disappear and the disintegration of cell were found in experimental group with HE staining;And it was almost normal in control group. A lot of cell underwent necrosis after 40 minutes irradiation in experimental group with AO-EB staining. Conclusion TiO2 film has photocatalytic killing effect on bovine LEC. The mechanism of cell death is mainly related to cell necrosis, which the TiO2 under UVA light irradiation produced the reactive oxygen species on cell surface and the cells were then killed by the action of these highly oxidizing species.

关 键 词：上皮细胞 晶状体 二氧化钛 光催化剂膜 

分 类 号：R776[医药卫生—眼科]