H SP27和H SP70在老年性白内障和透明晶状体中的表达  

Expression of HSP27 and HSP70 in age-related cataract and normal lens

作　　者：张伟英[1] 汪浩[1] 宋蕾[1] 茅惠明[2] 

机构地区：[1]同济大学附属东方医院眼科,上海200120 [2]同济大学附属东方医院中心实验室,上海200120

出　　处：《同济大学学报（医学版）》2008年第3期33-37,共5页Journal of Tongji University(Medical Science)

基　　金：上海市浦东新区社会发展局卫生科技基金资助项目(PW2003-B7)

摘　　要：目的观察热休克蛋白HSP27和HSP70在老年性白内障和透明晶状体中的表达情况。方法WesternBlot法测定HSP27和HSP70在老年性白内障和透明晶状体中的表达情况。结果老年性白内障的晶状体和透明晶状体中均有HSP27和HSP70的表达。经t检验,老年性白内障晶状体中HSP27(1.08±0.33)的表达与正常对照组(0.61±0.13)相比,差异存在统计学意义(P<0.05),老年性白内障晶状体中HSP70(1.41±0.48)的表达与正常对照组(0.67±0.12)相比,差异亦存在统计学意义(P<0.05)。结论老年性白内障晶状体中HSP27和HSP70的表达高于透明晶状体。Objective To study the expression of heat shock protein 27 （HSP27） and heat shock protein 70 （HSP70） in human age-related cataract lens and normal lens. Methods The expression of HSP27 and HSP70 in human age-related cataract lens and normal lens were measured by Western Blot. Results Both HSP27 and HSP70 were founded in human age-related cataract lens and normal lens. The expression of HSP27 （1.08±0.33 ） in age-related cataract lens were higher than those in normal lens （0.61±0.13, P 〈 0.05 ）. The expression of HSP70 （ 1.41±0.48 ） in age-related cataract lens were higher than those in normal lens（0.67±0.12, P 〈 0.05 ）. Conclusion Expression of HSP27 and HSP70 in age-related cataract lens are higher than those in normal lens.

关 键 词：热休克蛋白 HSP70 HSP27 白内障 

分 类 号：R776.1[医药卫生—眼科]