转化生长因子β受体Ⅰ、Ⅱ和Smad 4在胃癌中的表达及其临床意义  

Expression of transforming growth factor βreceptor Ⅰ,and Smad 4 mRNA and their clinical significance in gastric cancer

作　　者：沈迎春[1] 刘华[1] 

机构地区：[1]同济大学附属第十人民医院消化内科,上海200072

出　　处：《同济大学学报（医学版）》2008年第3期79-83,共5页Journal of Tongji University(Medical Science)

基　　金：上海市卫生局青年科研基金资助项目(20064650)

摘　　要：目的胃癌是世界上最为常见的恶性肿瘤之一,预后较差,是人类主要的肿瘤致死病因。本研究旨在探讨转化生长因子β受体Ⅰ、Ⅱ(TβRⅠ、Ⅱ)和Smad 4 mRNA与胃癌发生、发展的关系。方法采用免疫组织化学和分子原位杂交的方法对67例胃癌、24例正常胃粘膜的TGFβ1蛋白和Smad 4 mRNA表达进行检测。结果胃癌组织中TβRⅠ、Ⅱ蛋白和Smad 4 mRNA表达率(分别为73.13%、49.25%和44.78%)较正常黏膜(均为95.83%)相比,均明显减弱(P<0.05)。TβRⅠ蛋白的表达与胃癌的临床病理因素无关(P>0.05),TβRⅡ蛋白和Smad 4 mRNA在胃癌中表达的变化与胃癌的临床病理因素有关(P<0.05)。TβRⅡ蛋白和Smad 4 mRNA在胃癌中表达的变化呈明显的负相关(P<0.05)。结论TβRⅡ蛋白和Smad 4 mRNA的低表达对细胞的恶性转化及增殖有一定的生物学意义。Objective Gastric cancer, the major cause of carcinoma death, is one of the most common malignant tumors in the world, which has a very poor prognosis. To evaluate the significance of transforming growth factor βreceptor （ TβR Ⅰ , Ⅱ ） protein and Smad 4 mRNA in pathogenisis and development of gastric cancer. Methods The TβR Ⅰ, Ⅱ protein and Smad 4 mRNA were detected respectively by immunohistochemistry and hybridization in situ （cDNA-mRNA） in 67 gastric cancer tissues and 24 normal gastric mucosa. Results About TβR Ⅰ, Ⅱ protein and Smad 4 mRNA, there was a significantly lower positive rate in gastric carcinoma（73.13% ,49.25% and 44.78% ,respectively） than that in normal gastric mucosa （ they are all 95.83% ）, （ P 〈 0.05 ）. Expression of TβR Ⅱ protein and Smad 4 mRNA in gastric cancer tissues show a strong relationship with the, but there shows no relationship between expression of TβR Ⅰ protein and clinicopathologocal factors. There exist a negative correlation of the positive expression between TβR Ⅱ and Smad 4 （ P 〈 0.05）. Conclusion TβR Ⅱ protein and Smad 4 mRNA are involved in the gastric carcinoma cells proliferation and malignant transformation.

关 键 词：胃肿瘤 转化生长因子β受体Ⅰ 转化生长因子β受体Ⅱ SMAD4MRNA 免疫组织化学 原位杂交 

分 类 号：R735.2[医药卫生—肿瘤]