离子色谱法测定荔枝核及其732树脂洗脱液的游离氨基酸  

DETERMINATION OF FREE AMINO ACID OF LITCHI FRUIT STONE AND ITS ELUTE FROM 732 RESIN BY ION CHROMATOGRAPHY

作　　者：王洋 黄雪松[1] 

机构地区：[1]暨南大学理工学院食品科学与工程系,广东广州510632

出　　处：《食品研究与开发》2008年第6期106-110,共5页Food Research and Development

摘　　要：为测定荔枝核中的游离氨基酸,采用ICS-2500离子色谱分析仪,分别测定了荔枝核提取液与732阳离子交换树脂洗脱液中的氨基酸的组成和含量。离子色谱条件:固定相为Amino PACPA-10离子交换柱,流动相:250 mmol/L NaOH、1 mol/L NaAC和水作为洗脱液进行梯度洗脱,柱温30℃,流速0.25 mL/min。在稀释100倍的荔枝核提取液中含有12种游离氨基酸,其中苏氨酸、精氨酸和甘氨酸总相对含量为64%,且含有3种未知氨基酸,但未知氨基酸不稳定,离子交换树脂未起到预期的分离效果。因此,离子色谱法可有效的检测荔枝核中及分离纯化过程中的游离氨基酸。其中未知氨基酸的适宜分离条件有待进一步研究。Free amino acids＇ s content and composition in Litchi fruit stone＇ s extraction and eluate by 732 cation exchange resin were determined by ICS-2500 Ion chromatograph. Ion chromatographic condition was as follows： column was Amino PAC PA-10 ion exchange resin, using 250 mmol/L NaOH,1 mol/L NaAC and water as eluate for gradient elution, and the temperature of the column was 30 ℃ ,finally the velocity was 0.25 mL/min. There are 12 kinds of free amino acid in Litchi fruit stone extract diluted 100 times, among of these, the relative content of threonine, arginine and glycin was 64 %. And there are three of the 12 were unknown and volatile , the anticipative separation effect of ion exchange resin did not come true. Ion chromatography could detect the free amino acid in Litchi fruit stone and the process of separation and purification effectively. But the feasible separation conditions of unknown amino acid should be studied further.

关 键 词：荔枝核 氨基酸 离子色谱 阳离子交换树脂 

分 类 号：TS255[轻工技术与工程—农产品加工及贮藏工程]