^(125)I放射性粒子对体外培养的食管癌Eca-109细胞株克隆形成的影响  

Effects of ^(125)I radioactive seed on cloning efficiency of esophageal carcinoma cell line Eca-109 in vitro

作　　者：肖建[1] 曹秀峰[1] 龚涌灵[1] 曹国宪[2] 俞惠新[2] 谭成[2] 

机构地区：[1]南京医科大学附属南京第一医院肿瘤中心,江苏南京210006 [2]江苏省原子医学研究所卫生部重点核医学实验室,江苏无锡214036

出　　处：《现代肿瘤医学》2008年第6期883-885,共3页Journal of Modern Oncology

基　　金：卫生部临床科研协作研究基金资助项目子课题(编号:WKJ2005-3-006)

摘　　要：目的:研究125I放射性粒子对体外培养的人食管癌Eca-109细胞克隆形成率的影响。方法:取高糖型DMEM培养的对数生长的人食管癌Eca-109细胞,制成单细胞悬液,细胞计数并稀释,接种于35mm直径培养皿。设置A组:对照组(不加粒子),B组:低剂量实验组(加入0.2mCi125I粒子1枚),C组:中剂量实验组(加入0.4mCi125I粒子1枚),D组:高剂量实验组(加入0.8mCi125I粒子1枚),37℃、5%CO2恒温培养箱进行培养,于培养后第1-7日进行各组克隆计数并计算克隆形成率。结果:1周后A、B、C、D各组克隆形成率分别为72.73%、57.84%、34.41%、22.35%,实验组各组细胞克隆形成率均低于对照组,差别有统计学意义(P<0.05);C组与B组,D组与B组比较,有显著统计学意义(χ2=10.73,P<0.01;χ2=24.02,P<0.01);D组与C组比较,差别无统计学意义(χ2=3.16,P>0.05)。结论:125I放射性粒子可以降低体外培养的人食管癌Eca-109细胞克隆形成率。Objective：To determine the effects of ^125Ⅰ radioactive seed on cloning efficiency of human esophageal carcinoma cell line Eca -109 in vitro. Methods： Human esophageal carcinoma cell line Eca - 109 in exponentially growing phase incubated in high - glucose DMEM was made into a single - cell suspension. The numbers of cells were counted and the cells were diluted. Be seeded on 35 - mm cell culture dishes. Randomized into 4 groups ： A ： control group （the untreated cell）. B： low -dose group （treated with 0.2mCi^125Ⅰ seed）. C： Middle -dose group （treated with 0.4mCi^125Ⅰseed）. D： high - dose group （ treated with 0.8mCi^125Ⅰseed）. All cultures were done at 37℃ with 5% CO2 in a humidified incubator. The cloning efficiency of the 4 groups was evaluated from the l to 7 day. Results：After 7 days,the cloning efficiency of the 4 groups were 72.73% ,57.84% ,34.41% ,22.35%, respectively. The cloning efficiency of the 3 treated group statistically was lower than that of control group （ P 〈 0.05 ）. That of C and B, D and B groups showed statistically significant difference （ X^2 = 10.73,P 〈 0.01 ; X^2 = 24.02, P 〈 0.01 ）. But the cloning efficiency of D and C groups did not demonstrate a statistical difference（X^2 = 3.16, P 〉 0.05 ）. Conclusion ：^125Ⅰ radioactive seed shows a positively inhibiting effect to the cloning efficiency of esophageal carcinoma cells Eca - 109 in vitro.

关 键 词：食管肿瘤 ECA-109细胞株 ^125Ⅰ放射性粒子 克隆形成率 

分 类 号：R730.23[医药卫生—肿瘤] R735.1[医药卫生—临床医学]