小细胞肺癌细胞系SBC-5蛋白质组双向凝胶电泳图谱的建立  

Establishment of two-dimensional gel electrophoresis profiles of human small cell lung cancer cell line SBC-5

作　　者：冯燕国[1] 张贺龙[1] 冯英明[1] 康艳霞[1] 王宏玫[1] 胡玲[1] 阮禹松[2] 

机构地区：[1]第四军医大学唐都医院肿瘤科,陕西西安710038 [2]西安交通大学生命科学与技术学院信息中心,陕西西安710049

出　　处：《现代肿瘤医学》2008年第6期886-889,共4页Journal of Modern Oncology

基　　金：陕西省自然科学基金资助项目(2004C2-28)

摘　　要：目的:建立人小细胞肺癌细胞系SBC-5蛋白质组双向凝胶电泳图谱。方法:用固相pH梯度双向凝胶电泳技术分离SBC-5细胞总蛋白质,凝胶银染显色,PDQuest图像分析系统分析,从凝胶中选取1个分离较好的蛋白质点,应用基质辅助激光解析离子化-飞行时间-质谱技术(MALDI-TOF-MS)和数据库搜索鉴定蛋白质。结果:获得了背景清晰、分辨率和重复性较好的双向凝胶电泳图谱,三块胶平均蛋白质点数为1116±64,匹配率达84%。蛋白质点分布以PI 4-7、相对分子质量20000-80000范围内最多。1个蛋白点通过质谱分析和数据库检索得到了初步的鉴定。结论:建立了人小细胞肺癌细胞系SBC-5蛋白质组双向电泳参考图谱,为其蛋白质组学的进一步研究奠定了基础,也为人小细胞肺癌蛋白质组表达数据库的建立提供了有意义的数据。Objective：To establish the two-dimensional gel electrophoresis （2 -DE） reference map of proteome in human small cell lung cancer （SCLC） cell line SBC -5. Methods： Two - DE was applied to separate the total proteins of SBC - 5 cells, which were then silver - stained in the gel. A well - seperated protein spot were selected from the gel by PDQuest analysis system. Matrix - assisted laser desorption/ionization time of flight - mass spectrometry （MALDI- TOF- MS） ,poptide mass fingerprinting （PMF） , and database searching were used to identify the protein spot. Results： Clear, well - resolved, reproducible 2 - DE profiles of proteome in SBC -5 cells were obtained. The average protein spots of 3 gels were 1116 ± 64 with an average matching rate of 84%. The spots distributed in the greatest density at the isoelectric points of 4 - 7 and relative molecular mass weight of 20 000 - 80 000. One of these proteins were identified by mass spectrometry and database queries. Conclusion： The 2 - DE profiles of proteome from SBC - 5 cells were established and lay the foundation of further proteomics research of SBC - 5 cells. These data will be useful for establishing human SCLC proteome database.

关 键 词：小细胞肺癌 SBC-5细胞 双向凝胶电泳 蛋白质组 

分 类 号：R730.48[医药卫生—肿瘤] R734.2[医药卫生—临床医学]