芦荟大黄素对人胃癌细胞HGC-27增殖周期及凋亡的影响  

Effects of aloeemodin on proliferation cycle and apoptotic of human stomach cancer cell line HGC-27

作　　者：南菁[1] 秦云霞[1] 刘晶[2] 赵颖娜[1] 付强[1] 赵华栋[1] 

机构地区：[1]第四军医大学唐都医院普通外科,陕西西安710038 [2]西安石油大学校医院,陕西西安710000

出　　处：《现代肿瘤医学》2008年第6期919-921,共3页Journal of Modern Oncology

摘　　要：目的:探讨芦荟大黄素对人胃癌细胞HGC-27细胞生长的抑制作用及其对细胞增殖周期和凋亡的影响。方法:采用甲基噻唑(MTT)比色法和生长曲线测定不同浓度芦荟大黄素在不同作用时间内对在体外培养HGC-27细胞增殖的影响,同时应用流式细胞术检测细胞增殖周期及凋亡率的变化。结果:芦荟大黄素可在G0/G1期阻滞人胃癌细胞HGC-27的增殖,使S期细胞比率降低;在一定范围内,芦荟大黄素的浓度越高、作用时间越长,对肿瘤细胞生长的抑制作用越强,凋亡率也越高。结论:芦荟大黄素能抑制人胃癌细胞HGC-27细胞生长,并诱导细胞凋亡而阻止细胞周期。Objective ：To study the suppressive role of aloeemodin on the growth and its effect on the proliferation cycle and apoptosis of human stomach cancer cell line HGC - 27. Methods： The survival rate and the inhibitory rate of HGC -27 ceil in vitro were detected by MTT colorimetrie assay and cell growth curve assay at different time points under different concentration of aloeemodin; the cell proliferation cycle and the apoptotic rate were examined with flow cytometry analysis. Results ： Aloeemodin inhibited the proliferation of HGC - 27 ceil at G0/G1 phase, decreased the cell ratio at S phase. It suppressed the growth of tumor ceils and raised the apoptotic rate in a concentration and time depending manner in a certain extent. Conclusion： Aloeemodin could suppress the proliferation of HGC -27 cell to induce apoptosis and block cell cycle.

关 键 词：芦荟大黄素 胃癌细胞HGC-27 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]