沙培林对胃癌SGC7901细胞体外作用的研究  

Study on the effects of Sapylin on human gastric cancer cell SGC7901 in vitro

作　　者：李宁[1] 李建[1] 吴克瑾[1] 费哲为[1] 欧敬民[1] 叶敏[1] 

机构地区：[1]上海交通大学附属新华医院普外科,上海200092

出　　处：《现代肿瘤医学》2008年第6期921-924,共4页Journal of Modern Oncology

基　　金：上海市科委基金资助项目(编号:05ZR14064)

摘　　要：目的:观察沙培林在体外对人胃癌SGC7901细胞的作用。方法:以不同浓度的沙培林(0、0.001KE/ml、0.01KE/ml、0.1KE/ml和0.5KE/ml)处理6h、12h、24h和48h后,噻唑蓝(MTT)比色法观察SGC7901细胞的生长情况;0.5 KE/ml沙培林作用于胃癌SGC7901细胞48h后,流式细胞仪检测细胞周期的变化;免疫组化染色观察药物处理前后的p53、p27、PCNA蛋白的表达。结果:沙培林对SGC7901胃癌细胞具有抑制增殖效应,并在一定范围呈现时间和浓度依赖性;沙培林(0.5KE/ml)作用48h后,周期检测发现SGC7901细胞G0/G1期细胞下降,S期增加(P<0.01);免疫组化结果显示:沙培林干预可使SGC7901细胞p53蛋白表达下调,p27蛋白表达上调(P<0.01),PCNA蛋白表达组间无统计学差异(P>0.01)。结论:沙培林可改变SGC7901细胞周期分布,抑制细胞增殖;可使细胞p53表达减少,p27表达增加。Objective ：To study the effects of Sapyhn on human gastric cancer cell SGC7901 in vitro. Methods： SGC7901 cells were treated with 0,0. 001,0.01,0.1 and 0.5 KE/ml Sapylin for6,12,24,48 hours respectively. The cell growth condition was observed by MTT assey ;The cell cycle was analyzed by flow cytometry ;Immunocytochemical staining was used to analyze the expression of p53, p27 and PCNA protein in SGC7901 cell between control group and treated group. Results： Sapyin inhibited the proliferation of SGC7901 cells by. dose-dependent and time -dependent manner. SGC7901 cells were treated with 0.5 KE/ml Sapylin for 48 hours ,cells in G0/G1 - phase were decreased in cell cycle, but increased in S -phase （P 〈 0.01 ）. Results of immunocytochemical staining showed that Sapylin can down- regulated the expression of p53 protein ,up-regulated the p27 protein （P 〈 0.01 ）. The expression of PCNA showed no significant difference between control group and treated group（ P 〉 0.01 ）. Conclusion ： Sapylin can change the cell cycle, inhibit the proliferation of human gastric cancer SGC7901cell. It can also increase the expression of p53 protein,but decrease the p27 protein.

关 键 词：沙培林 SGC7901细胞 胃癌 P53 P27 

分 类 号：R735.2[医药卫生—肿瘤]