N-乙酰氨基半乳糖转移酶2对胃癌细胞SGC7901黏附迁移能力的影响  

Polypeptide:N-acetylgalactosaminytransferases 2′s role during adhesion and migration of gastric cancer cell SGC7901 at cell level

作　　者：岳爱环[1] 行书丽[1] 唐春花[1] 徐岚[1] 吴士良[1] 

机构地区：[1]苏州大学基础医学与生物科学学院生化与分子生物学教研室生化工程研究所,江苏苏州215123

出　　处：《江苏大学学报（医学版）》2008年第3期223-226,I0002,共5页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(30670462)

摘　　要：目的:探讨N-乙酰氨基半乳糖转移酶2(Polypeptide:N-acetylgalactosam inytransferases 2,ppGalNAc-T2)对胃癌细胞SGC7901黏附迁移的影响。方法:将ppGalNAc-T2正义载体(pEGFP-C1-T2)、空载体(pEGFP-C1)及干扰载体(pS ilenC irc le)转染SGC7901细胞,采用反转录PCR方法检测其在mRNA水平表达,分别通过细胞黏附实验和穿膜实验检测其黏附力和迁移力变化。结果:转染正义ppGalNAc-T2的SGC7901细胞比转染干扰载体的SGC7901细胞对纤连蛋白、基质胶和透明质酸的黏附能力低,但其侵袭迁移能力没有变化。结论:ppGalNAc-T2低表达可能与肿瘤浸润转移具有相关性。Objective ： To investigate Polypeptide ： N-acetylgalactosaminytransferases 2 （ ppGalNAc-T2）＇ s role during adhesion and migration of gastric cancer cell SGC7901. Methods： To transfect ppGalNAc-T2 plus sense vector （ pEGFP-C1-T2 ）, vacuity vector （ pEGFP-C1 ） and interference vector （ pSilenCircle ） to SGC7901. We detected express level of ppGalNAc-T2 by RT-PCR and detected the capability change of adhesion and migration by cell adhesion and Transwell essay. Results： Contrast to SGC7901 which interference vector, the one transfected ppGalNAc-T2 plus sense vector had low adhesion capability to matrigel, hyaluronic acid and fibronectin, but the same migration capability. Conclusion： It was possible that ppGal- NAc-T2＇s low expression and tumor adhesion and migration had their correlation.

关 键 词：N-乙酰氨基半乳糖转移酶2(ppGalNAc-T2) 胃癌细胞SGC7901 黏附 迁移 

分 类 号：R735.2[医药卫生—肿瘤] R730.21[医药卫生—临床医学]