BPI-1095对大鼠局灶性脑缺血后caspase-3蛋白表达的影响  

Effects of BPI-1095 on Caspase-3 in Middle Artery Occlusion Rats

作　　者：李菁晶[1] 毛淑静[2] 万虹[3] 历俊华[3] 翟晶[3] 刘丽萍[1] 王春雪[1] 王拥军[1] 

机构地区：[1]首都医科大学附属北京天坛医院神经内科,北京市100050 [2]北京护士学校药理组,北京市101149 [3]北京市神经外科研究所,北京市100050

出　　处：《中国康复理论与实践》2008年第5期427-429,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：北京市优秀人才基金资助项目(20061d0300416)

摘　　要：目的观察BPI-1095对大鼠脑缺血后神经元caspase-3蛋白表达的影响。方法大鼠大脑中动脉闭塞缺血模型(n=90),随机分成6组:BPI-1095大剂量组(240mg/kg)、BPI-1095中剂量组(80mg/kg)、BPI-1095小剂量组(27mg/kg)、阿司匹林组(80mg/kg)、安慰剂组、假手术组。分析梗死面积百分数并观察各组Caspas-3蛋白表达。结果BPI-1095大、中剂量组梗死面积百分数小于安慰剂组(P<0.05);各组脑组织缺血周边和皮层caspase-3表达阳性细胞较安慰剂组减少,大剂量组caspase-3阳性细胞减少最多(P<0.05)。结论BPI-1095对大鼠永久性大脑中动脉闭塞模型的局灶脑缺血有神经保护作用,其作用机制与下调凋亡相关蛋白caspase-3的表达有关。Objective To investigate the effect of BPI-1095 on caspase-3 protein expression in middle artery occlusion （MCAO） rats. Methods Cerebral ischemia was induced with MCAO in adult male SD rats. Rats were randomly subjected into 6 groups with 15 rats in each group. Each rat has been given tested medication of different dosage and was sacrificed 24 h after treatment. The area of infarction was measured on each slice by image analysis system. Meanwhile, immunohistochemistry staining was used to identify caspase-3 expression in ischemic brain tissue. Results The infarcted area were significantly decreased in big and moderate dose treated rats （P〈0.05, vs the placebo group）. The expression of caspase-3 protein decreased in contralateral and ipsilateral hemisphere are as. The caspase-3 positive cell was significantly decreased in rats treated with big doses compared with placebo- or ASA treated rats. Conclusion BPI-1095 shows neuroprotection in MCAO rats, which may related with the inhibition of caspase-3 expression resulting in apoptosis in penumbra.

关 键 词：脑缺血 大脑中动脉闭塞(MCAO) CASPASE-3 细胞凋亡 缺血半暗带 

分 类 号：R743.3[医药卫生—神经病学与精神病学]