STR-PCR半定量检测嵌合体方法在非清髓异基因造血干细胞移植中的应用  

SEMI-QUANTITATIVE ANALYSIS OF CHIMERISM AFTER NON-MYELOABLATIVE ALLOGENEIC STEM CELL TRANSPLANTATION BY STR-PCR

作　　者：熊辉霞[1] 陈宝安[2] 

机构地区：[1]青海大学附属医院 [2]东南大学附属中大医院

出　　处：《青海医学院学报》2008年第1期54-57,共4页Journal of Qinghai Medical College

摘　　要：目的应用STR-PCR半定量嵌合体检测方法监测非清髓异基因造血干细胞移植(non-myeloablative allogeneic stem cell tansplantation,NAST)后嵌合体。方法采用短串联重复序列-聚合酶链反应(STR-PCR)结合聚丙烯酰胺凝胶电泳和银染色以及凝胶成像分析技术对28例接受NAST的患者进行嵌合体监测。结果27例患者形成造血细胞嵌合体,完全嵌合(completechimerism,CC)22例,混合嵌合(mixed chimerism,MC)5例;其中低比例嵌合(low level of mixed chi-merism,LMC)3例,高比例嵌合(high level of mixed chimerism,HMC)2例。稳定植入的患者+7天即达HMC,3例患者复发前检测到供体细胞嵌合率(donor cell,DC)下降,1例在CC状态下复发,1例LMC发生早期移植排斥,长期存活者均保持稳定CC。嵌合体监测下早期接受临床干预治疗的MC患者转化为CC并延长了无病生存期。结论STR-PCR是一种简单、敏感、经济的监测非清髓移植后嵌合体的方法。Objective To investigate the value of semi-quantitative analysis of chimerism after non-myeloablative allogeneic stem cell tansplantation（NAST） by short tandem repeat-polymerase chain reaction（STR-PCR）.Methods 28 patients received NAST were evaluated.Semi-quantitative assessment of hematopoietic chimerism was performed by STR-PCR,polyacrylamide gels,silver staining and detected by Image Analysis System.Results 27 cases formed hematopoietic chimerism,22 cases formed complete chimerism（CC） and 5 cases were of mixed chimerism（MC）,3 cases formed low level of mixed chimerism（LMC） and 2 cases were high level of mixed chimerism（HMC）.Patients with stable engraftments showed HMC on day ＋7.Before the time of relapse,STR-PCR indicated decrease of donor cell（DC） in 3 patients. One patient relapsed in CC status and another patient with LMC rejected grafts earlier.Patients with persistent CC were associated with a longer disease-free time.Patients received an early intervention of clinical treatment transferred from MC to CC or got a longer disease-free time.Conclusion STR-PCR provide a simple,sensitive and economic approach for monitoring of chimerism after transplantation.

关 键 词：短串联重复序列 非清髓造血干细胞移植 造血嵌合体 

分 类 号：Q81[生物学—生物工程] R733.7[医药卫生—肿瘤]