淮北市志贺菌毒力基因的研究  

Study on virulence gene of Shigella spp. in Huaibei City

作　　者：赵冬云[1] 黄其宇[1] 周锦英[1] 王敏[1] 徐艳丽[1] 胡万富[2] 胡守奎[2] 

机构地区：[1]安徽省淮北市疾病预防控制中心,安徽淮北235000 [2]安徽省疾病预防控制中心,合肥230061

出　　处：《中国卫生检验杂志》2008年第5期792-794,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的:对2006年安徽省淮北市菌痢项目分离到的52株志贺菌进行毒力基因的检测,以了解其毒力基因的携带情况。方法:应用PCR方法,以ipaH、ia1、setl、sen为靶基因进行检测,并用setl和sen对其进行基因分型。结果:52株志贺菌中,所有菌株的ipaH基因均为阳性,47株(90.38%)的ial基因为阳性,45株(86.54%)为set1阳性,38株(73.08%)为sen阳性。52株菌共分4种基因型:5株为setl(一)/sen(一),9株为setl(+)/sen(一),2株为setl(一)/sen(+),36株为setl(+)/sen(+)。结论:淮北市志贺菌的ShET-1和ShET-2携带率相当高,setl(+)/sen(+)为优势基因型。Objective：To understand the distribution of the virulence of Shigella spp.separated from Huaibei City in 2006.Methods：Detection of Shigella spp.by PCR assay targeting the ipaH gene,ial gene,set1 gene and sen gene,then genotype of Shigella spp.is make sure by set1 and sen.Results：A total of 52 strains of Shigella spp with ipaH gene were all positive,47 strains of Shigella spp.with ial gene（90.38%） were positive,45 strains of Shigella spp.with set1 gene（86.54%） were positive,38 strains of Shigella spp.with sen gene（73.08%） were positive.The 52 strains of Shigella spp.were divided into 4 genotypes：5 of set1（-）/sen（-）,9 of set1（＋）/sen（-）,2 of set1（-）/sen（＋）,36 of set1（＋）/sen（＋）.Conclusion：The carrier rate of Shigella spp.with ShET-1 and ShET-2 in Huaibei City is very high and set1（＋）/sen（＋） is the advantageous gene.

关 键 词：志贺菌 毒力基因 PCR 

分 类 号：R516.4[医药卫生—内科学]