CXCR2基因多态性与奶牛乳房炎和乳品质的关联  

Study on the relationships between polymorphisms of CXCR2 gene and milk quality and mastitis of dairy cow

作　　者：徐敏[1] 平富强[1] 陈仕毅[1] 赖松家[1] 刘益平[1] 

机构地区：[1]四川农业大学动物科技学院,雅安625014

出　　处：《遗传》2008年第4期463-468,共6页Hereditas（Beijing)

基　　金：国家"十一五"科技支撑计划课题(编号:2006BAD04A17)资助~~

摘　　要：采用PCR-SSCP技术研究了荷斯坦牛、西门塔尔牛和通江黄牛3个品种共160头个体CXCR2基因多态性与乳房炎抗性性状和对牛奶品质性状的遗传效应。结果表明:CXCR2基因有3个SNP多态位点,分别位于序列的第685bp、777bp和861bp位点,确定了5个等位基因A、B、C、E和F。685bp位点表现为BC和CC基因型,777bp位点表现为AA和AB基因型,861bp位点为CC和BC基因型。与奶牛乳房炎敏感性有关的基因型主要是BC、CC和FF,可能对乳房炎有抗性的是AA、AB和EE基因型。据不同基因型对乳品质的遗传效应分析来看,AA、AB和EE基因型的乳品质性状极显著或显著优于其他基因型。PCR-SSCP was applied to analyze the polymorphisms of CXCR2 gene and its relationships with milk quality and mastiffs in 160 cattle samples including Holstein dairy cow, Simmental dairy cow and Tongjiang cattle. The results showed that there were 3 SNP loci in CXCR2 gene, which located at 684 bp, 777 bp, 861 bp, respectively. Five alleles （A, B, C, E and F） were determined based on the 3 SNP loci. The genotypes for each locus were BC and CC, AA and AB, CC and BC at 685 bp, 777 bp and 861 bp, respectively. Results also demonstrated that genotypes BC, CC and FF were probably relevant with mastitis, but AA, BB and EE may resist the mastiffs of cattle. The genotypes AA, AB and EE may have better milk quality （extremely significant difference or significant difference） than other genotypes.

关 键 词：CXCR2基因 多态性 奶牛乳房炎 乳品质 

分 类 号：S858.23[农业科学—临床兽医学]