细胞外基质相关基因在大鼠肝再生中表达模式分析  

Expression profiles of the extracellular matrix-associated genes during rat liver regeneration

作　　者：李红蕾[1] 陈晓光[1] 张富春[1] 马纪[1] 徐存拴 

机构地区：[1]新疆大学生命科学与技术学院 [2]河南师范大学生命科学学院,新乡453007

出　　处：《遗传》2008年第3期333-340,共8页Hereditas（Beijing)

基　　金：国家重点基础研究发展计划(973项目)项目(编号:2006CB708506)资助~~

摘　　要：细胞外基质具有维持细胞极性、调节细胞粘附、增殖、组织器官形态、发生、分化等功能。为了进一步在基因转录水平了解细胞外基质在大鼠肝再生中变化和作用,用搜集网站资料和查阅相关论文等方法获得细胞外基质基因,用Rat Genome2302.0芯片检测它们在大鼠再生肝中表达情况,用真、假手术比较方法确定肝再生相关基因。初步证实上述97个基因与肝再生相关。其中,肝再生启动（部分肝切除（parital hepatectomy,PH）后0.5～4h）、G0/G1过渡（PH后4～6h）、细胞增殖（PH后6～66h）、细胞分化和组织结构功能重建（PH后72～168h）等4个阶段起始表达的基因数为49、19、73、5,基因总表达的次数为84、51、369、144,表明相关基因主要在肝再生启动阶段起始表达,在不同阶段发挥作用。它们表达的相似性分为均上调、上调占优势、均下调、下调占优势、上调和下调相近等5类,涉及38、21、21、10和7个基因,共上调411次,下调186次,分为24种表达模式,表明肝再生中细胞生理生化活动具有阶段性、多样性和复杂性。根据细胞外基质相关基因在肝再生中表达变化推测,肝再生前期纤粘连蛋白形成相关基因表达增强,肝再生中期胶原形成相关基因表达增强。It has been well known that the extracacellular matrix （ECM） plays an important role in cell polarization, cell adhesion, cell proliferation, morphogenesis and differentiation. For the sake of the further in-depth investigation of the changes and actions of ECM in liver regeneration （LR） at gene transcriptional level, the ECM-associated genes were obtained by databases searching and literature retrieving, subsequently their expression profiles in rat regenerating liver were detected using Rat Genome 230 2.0 array, then LR-associated genes were identified based on comparison of the gene expression difference between sham operation （SO） group and partial hepatectomy （PH） group. A total of 97 genes were verified to be LR-associated. The initially and totally expressed number of these genes occurring in initial phase of LR, G0/G1 transition, cell proliferation, cell differentiation and structure-functional reconstruction were 49, 19, 43, 5 and 84, 51,369, 144, respectively, illustrating that expression of the ECM-associated genes were initiated mainly in the early phase, working in different phases. Their expression similarity was classified into 5 groups including only up-, predominantly up-, only down-, predominantly down-, and equal in up-regulated and down-regulated, involving 38, 21, 21, 10 and 7 genes, respectively; the number of up-regulated expressed genes and down-regulated expressed ones was 411 and 186; their expression patterns were categorized into 24 types, showing that the physiological and biochemical activities in LR were characterized by phase, diversity and intricacy. According to expression profiles and expression patterns of the ECM-associated genes in LR, it was confirmed that the levels of the below-listed genes in expression increased at the corresponding phases of LR, including fibronectin-associated genes at early phase in LR, and collagen-associated genes at middle phase.

关 键 词：部分肝切除 RAT Genome2302.0芯片 细胞外基质 肝再生相关基因 

分 类 号：Q343[生物学—遗传学]