人静脉移植物MMP-2和Ⅰ、Ⅲ、Ⅵ型胶原纤维的表达  

Expression of MMP-2,typeⅠ,Ⅲ and Ⅵcollagens in human vein graft

作　　者：郭桂平[1] 王岐本[2] 黄庆红[2] Jutta Schaper 

机构地区：[1]中南大学湘雅公共卫生学院办公室,长沙410078 [2]湘南学院基础医学课部,郴州423000 [3]德国马普研究所实验心脏学研究室

出　　处：《中国临床解剖学杂志》2008年第3期318-320,共3页Chinese Journal of Clinical Anatomy

基　　金：国家自然科学基金(30540064)

摘　　要：目的:探讨人静脉移植物MMP-2(基质金属蛋白酶)和Ⅰ型、Ⅲ型、Ⅵ型胶原纤维的表达情况。方法:应用免疫荧光组织化学技术对30个静脉移植物再塞标本中MMP-2、Ⅰ型、Ⅲ型、Ⅵ型胶原纤维的表达进行了检测,用激光共聚焦显微镜拍片,图片用SiliconGraphicsOctane进行处理。结果:在正常静脉血管,有少量MMP-2的表达;Ⅰ型、Ⅲ型、Ⅵ型胶原的表达较少。在静脉移植的血管,MMP-2的表达显著增加,是正常血管的3.5倍;Ⅰ型、Ⅲ型、Ⅵ型胶原的表达明显上调,分别是正常血管的3.8倍、4.0倍和3.9倍。另外,统计分析表明上述蛋白质在新内膜的表达均显著高于在中膜的表达(P<0.01)。结论:在静脉移植物的新内膜,MMP-2的表达上调,细胞外基质Ⅰ型、Ⅲ型、Ⅵ型胶原合成增加,提示这些重要分子在新内膜的形成和静脉再狭窄的病理过程中具有重要的作用。Objective To investigate the expression of MMP-2, type Ⅰ, Ⅲ and Ⅵ collagens in human vein graft. Methods： Confocal immunofluoresence with specific antibodies against MMP, type Ⅰ , Ⅲ and Ⅵ collagens were use in detecting above proteins in 30 human vein grafts, The data was processed by Silicon Graphics Octanesystem. Results： In normal veins, expression of MMP-2,type Ⅰ , Ⅲ and Ⅵcollagens all were very low; In vein grafts, expression of MMP-2 was significantly increased, 3.5 times over that in normal vessels; Expression of type Ⅰ, Ⅲ and Ⅵcollagens was also signicicantly up-regulated, 3.8, 4.0 and 3.9 times higher than that in normal vessels, respectively （P〈0.01）. Conclusions： The data demonstrates that expression of MMP-2, type Ⅰ , Ⅲ and Ⅵ collagens in the neointima of human vein grafts significantly up-regulated, suggesting that these proteins play an important role in the pathology of restenosis.

关 键 词：MMP-2 胶原 静脉移植物 共聚焦免疫荧光术 

分 类 号：R363[医药卫生—病理学]