益气清热养阴中药对晚期肺癌恶病质小鼠TNF-α及其受体表达的影响  

Experiment research on TNF-α and TNFR in advanced lung cancer cachexia model with Benefiting qi clearing heat and nourishing yin Decoction

作　　者：杜秀平[1] 韩正祥[1] 高向阳[1] 史会昌[1] 

机构地区：[1]徐州医学院附属医院肿瘤科,江苏徐州221002

出　　处：《临床肿瘤学杂志》2008年第5期405-408,共4页Chinese Clinical Oncology

基　　金：江苏省科技厅自然科学发展计划基金课题(BS2004513);徐州市科技局基金资助课题(XZ200425-5)

摘　　要：目的:研究益气清热养阴中药对晚期肺癌恶病质小鼠肿瘤坏死因子-α(TNF-α)及其受体(TNFR1、TNFR2)表达的影响。方法:建立小鼠Lewis肺癌胸腔移植恶病质模型,随机分为6组:正常对照组(NC)、模型对照组(MC)、中药组(TCM)、消炎痛组(IND)、顺铂组(DDP)、甲羟孕酮组(MPA)。ELISA法检测恶病质小鼠血清中TNF-α水平变化;免疫组织化学法(IHC)检测肿瘤组织中TNF-α、TNFR1、TNFR2的表达情况。结果:除正常对照组外,中药组中TNF-α的血清浓度明显低于其他组(P<0.05)。肿瘤组织IHC:中药组TNF-α和TNFR1表达较其他组有不同程度减少(P<0.05);而TNFR2表达较其他组增加(P<0.05)。结论:益气清热养阴中药可能通过调节TNF-α、TNFR1和TNFR2的表达发挥抗癌性恶病质的作用。Objective：To research the expression of TNF-α and TNFR on the advanced Lewis lung cancer cachexia model with Benefiting qi clearing heat and nourishing yin decoction.Methods：The advanced lung cancer cachexia models were established,and divided into six groups randomly：normal control group,model control group,TCM group,IND group,DDP group,MPA group.The level of TNF-α,TNFR1 and TNFR2 were measured.Results：Except the normal control group,the TNF-α serum level of TCM group was lower than other groups.IHC：the expression of TNF-α and TNFR1 in TCM group was significantly lower than other groups（P〈0.05）.but TNFR2 was adverse（P〈0.05）.Conclusion：Benefiting qi clearing heat and nourishing yin decoction maybe improve the syndrome of cancer cachexia by regulating the expression of TNF-α and TNFR1.

关 键 词：癌性恶病质 中药 动物模型 肿瘤坏死因子-α 肿瘤坏死因子受体 

分 类 号：R730.52[医药卫生—肿瘤]