蛋白激酶C在结肠癌多细胞耐药中的实验研究  

The experimental study of PKC on multicellular resistance (MCR) of colorectal carcinoma HT-29 cells

作　　者：潘凤[1] 梁后杰[1] 彭亦良[1] 边志衡[1] 彭秋平[1] 

机构地区：[1]第三军医大学西南医院肿瘤科,重庆400038

出　　处：《临床肿瘤学杂志》2008年第5期432-435,共4页Chinese Clinical Oncology

基　　金：第三军医大学青年科研基金资助(XG200522)

摘　　要：目的:探讨蛋白激酶C(protein kinase C,PKC)在结肠癌细胞多细胞耐药中的作用。方法:采用体外三维细胞培养的方法,通过应用不同剂量PKC抑制剂Staurosporine(SP)对三维(3D)培养结肠癌HT-29细胞药物敏感性、核因子-κB(NF-κB)活性及PKC活性的影响的研究,探讨PKC在结肠癌细胞群集耐药中的作用。结果:3D培养结肠癌细胞中PKC、NF-κB活性较二维(2D)培养细胞明显增高,氟尿嘧啶(5-FU)的药物敏感性降低;SP不同浓度处理HT-29球形细胞可抑制其PKC及NF-κB活性,对5-FU的药物敏感性增加,在一定浓度范围内,随着SP浓度的增加,抑制作用逐渐增强,存在量效关系。结论:PKC在结肠癌细胞群集耐药中有一定作用,抑制PKC活性,可增加结肠癌球形细胞对5-FU的敏感性。Objective：To study the effects of protein kinase C（PKC）on multicellular resistance（MCR）of colorectal carcinoma HT-29 cells in vitro.Methods：HT-29 cells were cultured as three-dimensional model using liquid overlay technique or as monolayer using routine method.It was studied that the activities of PKC and NF-κB and the sensitivity to 5-FU in HT-29 spheroids by the PKC inhibitor Staurosporine（SP）treatment in different concentrations.Results：The activities of PKC and NF-κB in HT-29 spheroids were higher than that of monolayer.SP treatment in different concentrations not only could inhibit the activities of PKC and NF-κB but also could induce the sensitivity to 5-FU in HT-29 spheroids.The larger the dosage was used,the more intensifide inhibitive effct could be obtained.Conclusion：There are regulating mechanisms on multicellular resistance of colorectal carcinoma HT-29 cells by PKC in vitro,the inhibition of PKC could induce sensitivity to 5-FU in HT-29 spheroids.

关 键 词：蛋白激酶C 结肠癌 多细胞耐药 

分 类 号：R730.58[医药卫生—肿瘤] R735.35[医药卫生—临床医学]