膀胱癌组织中P-糖蛋白、多药耐药相关蛋白、Bad的表达及其与临床病理的关系  

Expression of P-glycoprotein,multidrug resistance protein and Bad in bladder carcinoma and its relation to clinicopathology

作　　者：须霆[1] 邹青[1] 汪仪俊[1] 杨尔炘[1] 

机构地区：[1]江苏省肿瘤医院泌尿外科,南京210009

出　　处：《临床肿瘤学杂志》2008年第5期446-449,共4页Chinese Clinical Oncology

摘　　要：目的:探讨膀胱癌组织中P-糖蛋白(P-gp)、多药耐药相关蛋白(MRP)和凋亡蛋白Bad的表达及其与临床病理之间的关系。方法:采用SP法检测67例膀胱癌组织及20例正常膀胱组织中P-gp、MRP和Bad的表达情况。结果:67例膀胱癌患者中P-gp、MRP和Bad的阳性率分别为65.7%(44/67)、58.2%(39/67)和62.7%(42/67),而在正常膀胱粘膜中分别为10.0%(2/20)、5.0%(1/20)和5.0%(1/20),二者比较均有统计学差异(P<0.05);35例初发膀胱癌组织中,P-gp、MRP和Bad阳性表达率分别为45.7%、48.6%、71.4%,与32例复发膀胱癌组织中的87.5%、68.8%、51.3%比较,亦均有显著性差异(P<0.05)。P-gp与MRP表达无明显相关性(P>0.05)。膀胱癌细胞分化程度越低,临床分期越高,P-gp阳性率越高(P<0.05),Bad表达越低,呈负相关关系。MRP与临床分期、组织分级无相关性。结论:P-gp过表达、Bad低表达在膀胱癌复发中可能具有一定的协同作用。Objective：To study the expression of P-glycoprotein（P-gp）,multidrug resistance protein（MRP）and Bad in transitional cell carcinoma of bladder（TCC）and normal bladder mucosa and its relation to clinicopathology.Methods：S-P immnohistochemical staining was performed to evaluate the positivity of P-gp,MRP and Bad in 67 cases of TCC and 20 cases of normal bladder mucosa.Results：The positive rate of P-gp,MRP and Bad was 65.7%（44/67）,58.2%（39/67）and 62.7%（42/67）in 67 cases of TCC,but 10.0%（2/20）,5.0%（1/20）and 5.0%（1/20）in 20 normal bladder mucosa（P〈0.05）respectively.Compared with the initial cases,the expression of P-gp had a higher level,and no relation with MRP,but the expression of Bad had a lower level in the relapse cases.The expression of P-gp and MRP had no correlation（P〉0.05）.But the expression of P-gp and Bad had negative correlation：The lower differentiation of the TCC,the higher grade of the clinical stage,the positive rate of the expression of P-gp was higher（P〈0.05）and Bad was lower.The expression of MRP had no relation between the clinic stage and differentiation of TCC.Conclusion：The overexpression of the protein of P-gp and lower expression of Bad had relation to recurrence of bladder.

关 键 词：膀胱肿瘤 P-糖蛋白 多药耐药相关蛋白 BAD 免疫组化 

分 类 号：R737.14[医药卫生—肿瘤]