RhoA在卵巢癌细胞SKOV3体外侵袭和迁移过程中的作用  

The effect of RhoA on SKOV3 cell invasion and migration in vitro

作　　者：赵良平[1] 王薇娜[2] 张庆华[1] 田训[1] 黄磊[1] 梁逢奇[1] 熊国平[1] 马丁[3] 

机构地区：[1]武汉市中心医院妇产科,武汉430014 [2]武汉大学中南医院心内科 [3]华中科技大学同济医学院附属同济医院妇产科

出　　处：《现代妇产科进展》2008年第5期321-324,共4页Progress in Obstetrics and Gynecology

基　　金：国家重点基础研究发展规划973基金资助目(No:2002CB513100)

摘　　要：目的:探讨RhoA在卵巢癌细胞SKOV3体外侵袭和迁移过程中的作用。方法:构建RhoA真核表达载体,以脂质体介导转染卵巢癌细胞SKOV3,用RT-PCR和Westernblot检测SKOV3RhoAmRNA及蛋白表达水平;Boyden小室体外侵袭实验和划痕实验检测细胞侵袭和迁移能力。结果:转染RhoA的实验组与转染空载体的对照组比较,RhoAmRNA的表达丰度(RhoA/GAPDH)为12.01±1.72,明显高于对照组的6.81±1.24;RhoA蛋白的表达水平(RhoA/β-actin)为4.81±0.56,亦明显高于对照组的3.02±0.32,两者差异都有统计学意义(P<0.05)。Boyden小室体外侵袭实验中实验组与对照组平均侵袭百分数分别为(47.60±1.47)%和(22.60±2.40)%(P<0.05),比较划痕后0h与24h的宽度差,实验组和对照组愈合百分比分别为(65±6.4)%和(54±5.5)%(P<0.05);表明转染RhoA后,细胞侵袭和迁移能力明显增强。结论:RhoA增强了卵巢癌细胞SKOV3体外侵袭和迁移能力。Objective：To investigate the effects of RhoA in SKOV3 cell invasion and migration in vitro. Methods：Lipofect-2000 was used for the transfer of RhoA into SKOV3 cells. The expressions of RhoA mRNA and protein in transfected SKOV3 were detected respectively by reverse transcription-polymerase chain reaction （RT-PCR） and Western blot. Boyden chamber in vitro invasion assay and wound-healing assay were used to evaluate the invasive and migratory capability of SKOV3. Results ：The transfer of RhoA into SKOV3 resulted in ： （ 1 ） the increased expression of mRNA and protein of RhoA in transfected SKOV3 obviously （ RhoA mRNA/GAPDH 12.01 ±1.72 vs 6.81 ± 1.24;RhoA protein/β-actin 4.81 ±0.56 vs 3.02 ±0.32. P 〈0.05）, （2） the enhanced invasion and migration capacity in vitro. The percentage of invasion and wound closure in RhoA transfected cells were significantly higher compared those of vector transfectd cells [ （47.60 ±1.47）% vs （22.60 ±2.40）% ;（65 ±6.4）% vs （54 ±5.5）% ;P 〈0. 05]. Conclusion：RhoA transfection may increase the invasion and migration capacity of SKOV3 in vitro.

关 键 词：RHO因子 RHOA GTP结合蛋白 卵巢肿瘤 细胞系SKOV3 迁移 细胞 

分 类 号：R737.33[医药卫生—肿瘤]